NMR Analysis and Sequence of Toxin II from the Sea Anemone Radianthus paumotensis

David E. Wemmer; N. Vasant Kumar; Robert M. Metrione; Michel Lazdunski; Gary Drobny; Neville R. Kallenbach

doi:10.1021/bi00370a017

NMR Analysis and Sequence of Toxin II from the Sea Anemone Radianthus paumotensis

David E. Wemmer, N. Vasant Kumar, Robert M. Metrione, Michel Lazdunski, Gary Drobny, Neville R. Kallenbach

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Toxin II from Radianthus paumotensis (Rp_u) has been investigated by high-resolution NMR and chemical sequencing methods. Resonance assignments have been obtained for this protein by the sequential approach. NMR assignments could not be made consistent with the previously reported primary sequence for this protein, and chemical methods have been used to determine a sequence with which the NMR data are consistent. Analysis of the 2D NOE spectra shows that the protein secondary structure is comprised of two sequences of β-sheet, probably joined into a distorted continuous sheet, connected by turns and extended loops, without any regular a-helical segments. The residues previously implicated in activity in this class of proteins, D8 and R13, occur in a loop region.

Original language	English (US)
Pages (from-to)	6842-6849
Number of pages	8
Journal	Biochemistry
Volume	25
Issue number	22
DOIs	https://doi.org/10.1021/bi00370a017
State	Published - Nov 1986

ASJC Scopus subject areas

Biochemistry

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title = "NMR Analysis and Sequence of Toxin II from the Sea Anemone Radianthus paumotensis",

abstract = "Toxin II from Radianthus paumotensis (Rpu) has been investigated by high-resolution NMR and chemical sequencing methods. Resonance assignments have been obtained for this protein by the sequential approach. NMR assignments could not be made consistent with the previously reported primary sequence for this protein, and chemical methods have been used to determine a sequence with which the NMR data are consistent. Analysis of the 2D NOE spectra shows that the protein secondary structure is comprised of two sequences of β-sheet, probably joined into a distorted continuous sheet, connected by turns and extended loops, without any regular a-helical segments. The residues previously implicated in activity in this class of proteins, D8 and R13, occur in a loop region.",

author = "Wemmer, {David E.} and Kumar, {N. Vasant} and Metrione, {Robert M.} and Michel Lazdunski and Gary Drobny and Kallenbach, {Neville R.}",

year = "1986",

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language = "English (US)",

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pages = "6842--6849",

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T1 - NMR Analysis and Sequence of Toxin II from the Sea Anemone Radianthus paumotensis

AU - Wemmer, David E.

AU - Kumar, N. Vasant

AU - Metrione, Robert M.

AU - Lazdunski, Michel

AU - Drobny, Gary

AU - Kallenbach, Neville R.

PY - 1986/11

Y1 - 1986/11

N2 - Toxin II from Radianthus paumotensis (Rpu) has been investigated by high-resolution NMR and chemical sequencing methods. Resonance assignments have been obtained for this protein by the sequential approach. NMR assignments could not be made consistent with the previously reported primary sequence for this protein, and chemical methods have been used to determine a sequence with which the NMR data are consistent. Analysis of the 2D NOE spectra shows that the protein secondary structure is comprised of two sequences of β-sheet, probably joined into a distorted continuous sheet, connected by turns and extended loops, without any regular a-helical segments. The residues previously implicated in activity in this class of proteins, D8 and R13, occur in a loop region.

AB - Toxin II from Radianthus paumotensis (Rpu) has been investigated by high-resolution NMR and chemical sequencing methods. Resonance assignments have been obtained for this protein by the sequential approach. NMR assignments could not be made consistent with the previously reported primary sequence for this protein, and chemical methods have been used to determine a sequence with which the NMR data are consistent. Analysis of the 2D NOE spectra shows that the protein secondary structure is comprised of two sequences of β-sheet, probably joined into a distorted continuous sheet, connected by turns and extended loops, without any regular a-helical segments. The residues previously implicated in activity in this class of proteins, D8 and R13, occur in a loop region.

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ER -

NMR Analysis and Sequence of Toxin II from the Sea Anemone Radianthus paumotensis

Abstract

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