NMR delineation of inner and outer protons from paramagnetic relaxation perturbations in 1D and 2D spectra of peptides

Neri Niccolai; Alessandra Bonci; Mauro Rustici; M. Scarselli; Paolo Neri; Gennaro Esposito; Paolo Mascagni; Andrea Motta; Henríette Molinari

doi:10.1039/p29910001453

NMR delineation of inner and outer protons from paramagnetic relaxation perturbations in 1D and 2D spectra of peptides

Neri Niccolai, Alessandra Bonci, Mauro Rustici, M. Scarselli, Paolo Neri, Gennaro Esposito, Paolo Mascagni, Andrea Motta, Henríette Molinari

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The use of soluble spin labels to filter out the cross-peaks of outer proton nuclei in 2D NMR spectra is proposed as a general method of obtaining structural information about complex molecules. Gramicidin S, a decapeptide of well defined and stable structure, has been used as a model system to correlate the paramagnetic effects observed in 1D and 2D spectra and the peptide solution conformation. The method appears particularly suited to obtaining information about the hydrogen bonding of backbone amide protons.

Original language	English (US)
Pages (from-to)	1453-1457
Number of pages	5
Journal	Journal of the Chemical Society, Perkin Transactions 2
Issue number	9
DOIs	https://doi.org/10.1039/p29910001453
State	Published - 1991

ASJC Scopus subject areas

General Chemistry

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abstract = "The use of soluble spin labels to filter out the cross-peaks of outer proton nuclei in 2D NMR spectra is proposed as a general method of obtaining structural information about complex molecules. Gramicidin S, a decapeptide of well defined and stable structure, has been used as a model system to correlate the paramagnetic effects observed in 1D and 2D spectra and the peptide solution conformation. The method appears particularly suited to obtaining information about the hydrogen bonding of backbone amide protons.",

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year = "1991",

doi = "10.1039/p29910001453",

language = "English (US)",

pages = "1453--1457",

journal = "Journal of the Chemical Society, Perkin Transactions 2",

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T1 - NMR delineation of inner and outer protons from paramagnetic relaxation perturbations in 1D and 2D spectra of peptides

AU - Niccolai, Neri

AU - Bonci, Alessandra

AU - Rustici, Mauro

AU - Scarselli, M.

AU - Neri, Paolo

AU - Esposito, Gennaro

AU - Mascagni, Paolo

AU - Motta, Andrea

AU - Molinari, Henríette

PY - 1991

Y1 - 1991

N2 - The use of soluble spin labels to filter out the cross-peaks of outer proton nuclei in 2D NMR spectra is proposed as a general method of obtaining structural information about complex molecules. Gramicidin S, a decapeptide of well defined and stable structure, has been used as a model system to correlate the paramagnetic effects observed in 1D and 2D spectra and the peptide solution conformation. The method appears particularly suited to obtaining information about the hydrogen bonding of backbone amide protons.

AB - The use of soluble spin labels to filter out the cross-peaks of outer proton nuclei in 2D NMR spectra is proposed as a general method of obtaining structural information about complex molecules. Gramicidin S, a decapeptide of well defined and stable structure, has been used as a model system to correlate the paramagnetic effects observed in 1D and 2D spectra and the peptide solution conformation. The method appears particularly suited to obtaining information about the hydrogen bonding of backbone amide protons.

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JO - Journal of the Chemical Society, Perkin Transactions 2

JF - Journal of the Chemical Society, Perkin Transactions 2

IS - 9

ER -

NMR delineation of inner and outer protons from paramagnetic relaxation perturbations in 1D and 2D spectra of peptides

Abstract

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