NMR spectroscopy approach to study the structure, orientation, and mechanism of the multidrug exporter EmrE

Maureen Leninger, Nathaniel J. Traaseth

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Multidrug exporters are a class of membrane proteins that remove antibiotics from the cytoplasm of bacteria and in the process confer multidrug resistance to the organism. This chapter outlines the sample preparation and optimization of oriented solid-state NMR experiments applied to the study of structure and dynamics for the model transporter EmrE from the small multidrug resistance (SMR) family.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages83-96
Number of pages14
ISBN (Print)9781493974542
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1700
ISSN (Print)1064-3745

Keywords

  • Bicelles
  • Membrane proteins
  • Multidrug resistance
  • NMR pedagogy
  • PISEMA
  • Solid-state NMR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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