TY - GEN
T1 - Non-Contact Microfluidic Mixing Probe for Generating Controlled Concentration Gradient
AU - Ali, Dima S.
AU - Glia, Ayoub
AU - Sukumar, Pavithra
AU - Deliorman, Muhammedin
AU - Qasaimeh, Mohammad A.
N1 - Publisher Copyright:
© 2023 IEEE.
PY - 2023
Y1 - 2023
N2 - Concentration gradients influence a range of biological phenomena, such as human development, wound healing, inflammation, and cancer metastasis. Reconstructing and studying these gradients in vitro enables investigation of cellular responses and behaviors including cellular patterning, differentiation, migration, and resistance to drugs. In this work, we develop an open (non-contact) microfluidic device, named the microfluidic mixing probe (MMP). The MMP integrates the concept of microfluidic probes (MFPs) with microfluidic diffusion-based micromixers. The MMP, fully 3D printed using a stereolithography printer, is capable of generating gradients with 3 distinct concentrations: 100%, 50%, and 0%. On the substrate, these concentrations are confined within 3 individual hydrodynamic flow confinements. The generated concentration gradients are particularly useful for performing drug testing and chemotaxis studies on individual cells, including neutrophils, cancer cells, and neurons. The MMP is also capable of operating in 2D scanning mode, which allows for systematically maintaining the gradient for specific durations of time, and thus testing cell responses to spatiotemporal stimulations at single-cell levels.
AB - Concentration gradients influence a range of biological phenomena, such as human development, wound healing, inflammation, and cancer metastasis. Reconstructing and studying these gradients in vitro enables investigation of cellular responses and behaviors including cellular patterning, differentiation, migration, and resistance to drugs. In this work, we develop an open (non-contact) microfluidic device, named the microfluidic mixing probe (MMP). The MMP integrates the concept of microfluidic probes (MFPs) with microfluidic diffusion-based micromixers. The MMP, fully 3D printed using a stereolithography printer, is capable of generating gradients with 3 distinct concentrations: 100%, 50%, and 0%. On the substrate, these concentrations are confined within 3 individual hydrodynamic flow confinements. The generated concentration gradients are particularly useful for performing drug testing and chemotaxis studies on individual cells, including neutrophils, cancer cells, and neurons. The MMP is also capable of operating in 2D scanning mode, which allows for systematically maintaining the gradient for specific durations of time, and thus testing cell responses to spatiotemporal stimulations at single-cell levels.
KW - 3D printing
KW - Concentration gradient
KW - Microfluidic micro mixer
KW - Microfluidic probe
KW - Single cell
KW - Stimulation
UR - http://www.scopus.com/inward/record.url?scp=85178166119&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85178166119&partnerID=8YFLogxK
U2 - 10.1109/MARSS58567.2023.10294153
DO - 10.1109/MARSS58567.2023.10294153
M3 - Conference contribution
AN - SCOPUS:85178166119
T3 - Proceedings of MARSS 2023 - 6th International Conference on Manipulation, Automation, and Robotics at Small Scales
BT - Proceedings of MARSS 2023 - 6th International Conference on Manipulation, Automation, and Robotics at Small Scales
A2 - Haliyo, Sinan
A2 - Boudaoud, Mokrane
A2 - Qasaimeh, Mohammad A.
A2 - Fatikow, Sergej
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 6th International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2023
Y2 - 9 October 2023 through 13 October 2023
ER -