Non-contact small animal fluorescence imaging system for simultaneous multi-directional angular-dependent data acquisition

Jong Hwan Lee, Hyun Keol Kim, Chandhanarat Chandhanayingyong, Francis Young Lee, Andreas H. Hielscher

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We present a novel non-contact small animal fluorescent molecular tomography (FMT) imaging system. At the heart of the system is a new mirror-based imaging head that was designed to provide 360-degree measurement data from an entire animal surface in one step. This imaging head consists of two conical mirrors, which considerably reduce multiple back reflections between the animal and mirror surfaces. These back reflections are common in existing mirror-based imaging heads and tend to degrade the quality of raw measurement data. In addition, the introduction of a novel ray-transfer operator allows for the inclusion of the angular dependent data in the image reconstruction process, which results in higher image resolution. We describe in detail the system design and implementation of the hardware components as well as the transport-theorybased image reconstruction algorithm. Using numerical simulations, measurements on a well-defined phantom and a live animal, we evaluate the system performance and show the advantages of our approach.

    Original languageEnglish (US)
    Pages (from-to)2301-2316
    Number of pages16
    JournalBiomedical Optics Express
    Volume5
    Issue number7
    DOIs
    StatePublished - Jun 1 2014

    ASJC Scopus subject areas

    • Biotechnology
    • Atomic and Molecular Physics, and Optics

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