Nonphotochemical laser induced nucleation of hen egg white lysozyme crystal

In Sung Lee; James M.B. Evans; Deniz Erdemir; Alfred Y. Lee; Bruce A. Garetz; Allan S. Myerson

doi:10.1021/cg800696u

Nonphotochemical laser induced nucleation of hen egg white lysozyme crystal

In Sung Lee, James M.B. Evans, Deniz Erdemir, Alfred Y. Lee, Bruce A. Garetz, Allan S. Myerson

Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Small droplets of supersaturated hen egg white lysozyme (HEWL) solution were exposed to intense linearly polarized laser pulses with different wavelengths, intensities, and pulse durations. Laser irradiation under some conditions significantly increased the number of droplets in which crystals were observed in a given time period, compared with nonirradiated samples. As a general rule, nonphotochemical laser induced nucleation (NPLIN) in lysozyme solutions was more effective with shorter aging time, 532-nm wavelength, higher peak intensity, and shorter pulse duration. Bovine pancreatic trypsin (BPT) was also examined using NPLIN, showing the potential application of NPLIN to other proteins.

Original language	English (US)
Pages (from-to)	4255-4261
Number of pages	7
Journal	Crystal Growth and Design
Volume	8
Issue number	12
DOIs	https://doi.org/10.1021/cg800696u
State	Published - Dec 2008

ASJC Scopus subject areas

General Chemistry
General Materials Science
Condensed Matter Physics

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title = "Nonphotochemical laser induced nucleation of hen egg white lysozyme crystal",

abstract = "Small droplets of supersaturated hen egg white lysozyme (HEWL) solution were exposed to intense linearly polarized laser pulses with different wavelengths, intensities, and pulse durations. Laser irradiation under some conditions significantly increased the number of droplets in which crystals were observed in a given time period, compared with nonirradiated samples. As a general rule, nonphotochemical laser induced nucleation (NPLIN) in lysozyme solutions was more effective with shorter aging time, 532-nm wavelength, higher peak intensity, and shorter pulse duration. Bovine pancreatic trypsin (BPT) was also examined using NPLIN, showing the potential application of NPLIN to other proteins.",

author = "Lee, {In Sung} and Evans, {James M.B.} and Deniz Erdemir and Lee, {Alfred Y.} and Garetz, {Bruce A.} and Myerson, {Allan S.}",

year = "2008",

month = dec,

doi = "10.1021/cg800696u",

language = "English (US)",

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AU - Lee, In Sung

AU - Evans, James M.B.

AU - Erdemir, Deniz

AU - Lee, Alfred Y.

AU - Garetz, Bruce A.

AU - Myerson, Allan S.

PY - 2008/12

Y1 - 2008/12

N2 - Small droplets of supersaturated hen egg white lysozyme (HEWL) solution were exposed to intense linearly polarized laser pulses with different wavelengths, intensities, and pulse durations. Laser irradiation under some conditions significantly increased the number of droplets in which crystals were observed in a given time period, compared with nonirradiated samples. As a general rule, nonphotochemical laser induced nucleation (NPLIN) in lysozyme solutions was more effective with shorter aging time, 532-nm wavelength, higher peak intensity, and shorter pulse duration. Bovine pancreatic trypsin (BPT) was also examined using NPLIN, showing the potential application of NPLIN to other proteins.

AB - Small droplets of supersaturated hen egg white lysozyme (HEWL) solution were exposed to intense linearly polarized laser pulses with different wavelengths, intensities, and pulse durations. Laser irradiation under some conditions significantly increased the number of droplets in which crystals were observed in a given time period, compared with nonirradiated samples. As a general rule, nonphotochemical laser induced nucleation (NPLIN) in lysozyme solutions was more effective with shorter aging time, 532-nm wavelength, higher peak intensity, and shorter pulse duration. Bovine pancreatic trypsin (BPT) was also examined using NPLIN, showing the potential application of NPLIN to other proteins.

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Nonphotochemical laser induced nucleation of hen egg white lysozyme crystal

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