@article{eba5dccd87f848dab3b0c73b89cddf8e,
title = "Novel lotus-domain proteins are organizational hubs that recruit c. Elegans vasa to germ granules",
abstract = "We describe MIP-1 and MIP-2, novel paralogous C. elegans germ granule components that interact with the intrinsically disordered MEG-3 protein. These proteins promote P granule condensation, form granules independently of MEG-3 in the postembryonic germ line, and balance each other in regulating P granule growth and localization. MIP-1 and MIP-2 each contain two LOTUS domains and intrinsically disordered regions and form homo-and heterodimers. They bind and anchor the Vasa homolog GLH-1 within P granules and are jointly required for coalescence of MEG-3, GLH-1, and PGL proteins. Animals lacking MIP-1 and MIP-2 show temperature-sensitive embryonic lethality, sterility, and mortal germ lines. Germline phenotypes include defects in stem cell self-renewal, meiotic progression, and gamete differentiation. We propose that these proteins serve as scaffolds and organizing centers for ribonucleoprotein networks within P granules that help recruit and balance essential RNA processing machinery to regulate key developmental transitions in the germ line.",
keywords = "Biomolecular condensate, Embryogenesis, GLH-1, Germline, IDR, LOTUS, Liquid-liquid phase separation, OST-HTH, Oskar, P granules, Stem cell niche, Vasa",
author = "Cipriani, {P. Giselle} and Olivia Bay and John Zinno and Michelle Gutwein and Gan, {Hin Hark} and Mayya, {Vinay K.} and George Chung and Chen, {Jia Xuan} and Hala Fahs and Yu Guan and Duchaine, {Thomas F.} and Matthias Selbach and Fabio Piano and Gunsalus, {Kristin C.}",
note = "Funding Information: We thank Dr. K. Kionte (NYU, New York) for help with the production of CRISPR lines; P. Emhardt (NYUNY), F. Mohammed, and S. Gopinadhan (NYUAD) for technical assistance; Sean West for initial LOTUS domain identification; Rachid Rezgui (NYU Abu Dhabi Core Technology Platforms group) for help with Leica super-resolution microscopy; and Alan Twaddle and the NYU High Performance Computing team for computational support. We thank the members of the Gunsalus and Piano labs and Liz Marnik and Dustin Updike for helpful discussions and Tiffany Kilfeather for help with manuscript and figure preparation. This work was supported by a gift from NYU Abu Dhabi (to F.P.) and grants from the NYU Abu Dhabi Research Institute for the NYUAD CGSB (ADHPG CGSB to K.C.G.), the Canadian Institutes of Health Research (CIHR) MOP 123352 (T.F.D.), and the Charlotte and Leo Karassik Foundation (Ph.D. fellowship award to V.K.M.). J.X.C. received funding from the MDC-NYU PhD exchange program, supported by the BMBF (0315362) and the Berlin Institute for Medical Systems Biology (BIMSB). Some strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). Publisher Copyright: {\textcopyright} 2021, eLife Sciences Publications Ltd. All rights reserved.",
year = "2021",
month = jul,
doi = "10.7554/eLife.60833",
language = "English (US)",
volume = "10",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}