Nuclear Scaling Is Coordinated among Individual Nuclei in Multinucleated Muscle Fibers

Stefanie E. Windner, Angelika Manhart, Amelia Brown, Alex Mogilner, Mary K. Baylies

Research output: Contribution to journalArticlepeer-review

Abstract

Optimal cell performance depends on cell size and the appropriate relative size, i.e., scaling, of the nucleus. How nuclear scaling is regulated and contributes to cell function is poorly understood, especially in skeletal muscle fibers, which are among the largest cells, containing hundreds of nuclei. Here, we present a Drosophila in vivo system to analyze nuclear scaling in whole multinucleated muscle fibers, genetically manipulate individual components, and assess muscle function. Despite precise global coordination, we find that individual nuclei within a myofiber establish different local scaling relationships by adjusting their size and synthetic activity in correlation with positional or spatial cues. While myonuclei exhibit compensatory potential, even minor changes in global nuclear size scaling correlate with reduced muscle function. Our study provides the first comprehensive approach to unraveling the intrinsic regulation of size in multinucleated muscle fibers. These insights to muscle cell biology will accelerate the development of interventions for muscle diseases. Muscle fibers are large multinucleated cells with remarkable size plasticity. Windner et al. investigate the relationship between muscle cell size and nuclear content. They show that cells contain a heterogeneous population of nuclei and explore mechanisms of nuclear coordination, as well as the functional consequences of scaling perturbations.

Original languageEnglish (US)
Pages (from-to)48-62.e3
JournalDevelopmental Cell
Volume49
Issue number1
DOIs
StatePublished - Apr 8 2019

Keywords

  • Drosophila
  • Fibrillarin
  • cell size
  • nuclear positioning
  • nuclear scaling
  • nuclear synthetic activity
  • nucleolus
  • polyploidy
  • skeletal muscle
  • syncytial organization

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Nuclear Scaling Is Coordinated among Individual Nuclei in Multinucleated Muscle Fibers'. Together they form a unique fingerprint.

Cite this