On chip PCR amplification with integrated lateral flow detection

Zongyuan Chen, Jing Wang, Michael G. Mauk, Haim H. Bau, Cheryl Davis, Gary Tong, Frank Winslow, William R. Abrams, Dan Malamud

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A pneumatically-driven, microfluidic-based, disposable, polycarbonate cassette for DNA amplification and detection is described. The cassette consists of a monolithic - polycarbonate microfluidic chip and a lateral flow (LF) strip. The chip contains a polymerase chain reaction (PCR) chamber and a mixing/incubation chamber. The flow control was realized with phase-change valves that can operate in self-actuated mode and do not require any moving parts. The fusion and mixing of two liquids were achieved by pre-freezing the mixing chamber. Tests with B. cereus genomic DNA demonstrated that the performance of the cassette compared favorably with bench-top protocols.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1297-1299
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Keywords

  • LOC
  • Microfluidics
  • PCR-LF detection
  • Phase-change valve

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Fingerprint Dive into the research topics of 'On chip PCR amplification with integrated lateral flow detection'. Together they form a unique fingerprint.

  • Cite this

    Chen, Z., Wang, J., Mauk, M. G., Bau, H. H., Davis, C., Tong, G., Winslow, F., Abrams, W. R., & Malamud, D. (2005). On chip PCR amplification with integrated lateral flow detection. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1297-1299). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.