@inproceedings{1e9ea2f8f36f4a71b21acbb330e13508,
title = "On chip PCR amplification with integrated lateral flow detection",
abstract = "A pneumatically-driven, microfluidic-based, disposable, polycarbonate cassette for DNA amplification and detection is described. The cassette consists of a monolithic - polycarbonate microfluidic chip and a lateral flow (LF) strip. The chip contains a polymerase chain reaction (PCR) chamber and a mixing/incubation chamber. The flow control was realized with phase-change valves that can operate in self-actuated mode and do not require any moving parts. The fusion and mixing of two liquids were achieved by pre-freezing the mixing chamber. Tests with B. cereus genomic DNA demonstrated that the performance of the cassette compared favorably with bench-top protocols.",
keywords = "LOC, Microfluidics, PCR-LF detection, Phase-change valve",
author = "Zongyuan Chen and Jing Wang and Mauk, {Michael G.} and Bau, {Haim H.} and Cheryl Davis and Gary Tong and Frank Winslow and Abrams, {William R.} and Dan Malamud",
year = "2005",
language = "English (US)",
isbn = "0974361119",
series = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Transducer Research Foundation",
pages = "1297--1299",
booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference",
note = "9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 ; Conference date: 09-10-2005 Through 13-10-2005",
}