One and two codon insertion mutants of bacteriophage f1

Jef D. Boeke

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Simple methods for introducing one or two extra codons of genetic information into the f1 genome in vitro have been devised. The methods use various combinations of enzymes to insert three or six base-pairs into the RF DNA of the bacteriophage. Since such insertions do not cause frameshifts in coding regions, a number of these mutants are viable. Several such mutants were mapped and characterized. The methods described and variations of them can be applied to other circular DNA genomes.

    Original languageEnglish (US)
    Pages (from-to)288-291
    Number of pages4
    JournalMGG Molecular & General Genetics
    Volume181
    Issue number3
    DOIs
    StatePublished - Mar 1981

    ASJC Scopus subject areas

    • Genetics

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