TY - JOUR
T1 - Optical Control of a Biological Reaction–Diffusion System
AU - Glock, Philipp
AU - Broichhagen, Johannes
AU - Kretschmer, Simon
AU - Blumhardt, Philipp
AU - Mücksch, Jonas
AU - Trauner, Dirk
AU - Schwille, Petra
N1 - Funding Information:
P.G. is supported by GRK 2062—Molecular Principles of Synthetic Biology and would like to thank Peter Schultz, Beatrice Ramm, Leon Harrington, Stefan Pettera, and Henri Franquelim. J.M., P.B. and P.G. acknowledge support from the International Max Planck Research School for Molecular Life Sciences. J.B. would like to acknowledge SFB TRR 152 and David H. Woodmansee for providing precursors. D.T. acknowledges support from SFB TRR 152 and an ERC Advanced Grant. P.S. would like to acknowledge MaxSynBio.
Publisher Copyright:
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/2/23
Y1 - 2018/2/23
N2 - Patterns formed by reaction and diffusion are the foundation for many phenomena in biology. However, the experimental study of reaction–diffusion (R–D) systems has so far been dominated by chemical oscillators, for which many tools are available. In this work, we developed a photoswitch for the Min system of Escherichia coli, a versatile biological in vitro R–D system consisting of the antagonistic proteins MinD and MinE. A MinE-derived peptide of 19 amino acids was covalently modified with a photoisomerizable crosslinker based on azobenzene to externally control peptide-mediated depletion of MinD from the membrane. In addition to providing an on–off switch for pattern formation, we achieve frequency-locked resonance with a precise 2D spatial memory, thus allowing new insights into Min protein action on the membrane. Taken together, we provide a tool to study phenomena in pattern formation using biological agents.
AB - Patterns formed by reaction and diffusion are the foundation for many phenomena in biology. However, the experimental study of reaction–diffusion (R–D) systems has so far been dominated by chemical oscillators, for which many tools are available. In this work, we developed a photoswitch for the Min system of Escherichia coli, a versatile biological in vitro R–D system consisting of the antagonistic proteins MinD and MinE. A MinE-derived peptide of 19 amino acids was covalently modified with a photoisomerizable crosslinker based on azobenzene to externally control peptide-mediated depletion of MinD from the membrane. In addition to providing an on–off switch for pattern formation, we achieve frequency-locked resonance with a precise 2D spatial memory, thus allowing new insights into Min protein action on the membrane. Taken together, we provide a tool to study phenomena in pattern formation using biological agents.
KW - chemical oscillators
KW - optical control
KW - pattern formation
KW - photoswitches
KW - synthetic biology
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U2 - 10.1002/anie.201712002
DO - 10.1002/anie.201712002
M3 - Article
C2 - 29266672
AN - SCOPUS:85041017364
SN - 1433-7851
VL - 57
SP - 2362
EP - 2366
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 9
ER -