Optical control of GIRK channels using visible light

Julie B. Trads; Jessica Burgstaller; Laura Laprell; David B. Konrad; Luis De La Osa de La Rosa; C. David Weaver; Herwig Baier; Dirk Trauner; David M. Barber

doi:10.1039/c6ob02153k

Optical control of GIRK channels using visible light

Julie B. Trads, Jessica Burgstaller, Laura Laprell, David B. Konrad, Luis De La Osa de La Rosa, C. David Weaver, Herwig Baier, Dirk Trauner, David M. Barber

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

G-protein coupled inwardly rectifying potassium (GIRK) channels are an integral part of inhibitory signal transduction pathways, reducing the activity of excitable cells via hyperpolarization. They play crucial roles in processes such as cardiac output, cognition and the coordination of movement. Therefore, the precision control of GIRK channels is of critical importance. Here, we describe the development of the azobenzene containing molecule VLOGO (Visible Light Operated GIRK channel Opener), which activates GIRK channels in the dark and is promptly deactivated when illuminated with green light. VLOGO is a valuable addition to the existing tools for the optical control of GIRK channels as it circumvents the need to use potentially harmful UV irradiation. We therefore believe that VLOGO will be a useful research tool for studying GIRK channels in biological systems.

Original language	English (US)
Pages (from-to)	76-81
Number of pages	6
Journal	Organic and Biomolecular Chemistry
Volume	15
Issue number	1
DOIs	https://doi.org/10.1039/c6ob02153k
State	Published - 2017

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1039/c6ob02153k

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title = "Optical control of GIRK channels using visible light",

abstract = "G-protein coupled inwardly rectifying potassium (GIRK) channels are an integral part of inhibitory signal transduction pathways, reducing the activity of excitable cells via hyperpolarization. They play crucial roles in processes such as cardiac output, cognition and the coordination of movement. Therefore, the precision control of GIRK channels is of critical importance. Here, we describe the development of the azobenzene containing molecule VLOGO (Visible Light Operated GIRK channel Opener), which activates GIRK channels in the dark and is promptly deactivated when illuminated with green light. VLOGO is a valuable addition to the existing tools for the optical control of GIRK channels as it circumvents the need to use potentially harmful UV irradiation. We therefore believe that VLOGO will be a useful research tool for studying GIRK channels in biological systems.",

author = "Trads, {Julie B.} and Jessica Burgstaller and Laura Laprell and Konrad, {David B.} and {De La Osa de La Rosa}, Luis and Weaver, {C. David} and Herwig Baier and Dirk Trauner and Barber, {David M.}",

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year = "2017",

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TY - JOUR

T1 - Optical control of GIRK channels using visible light

AU - Trads, Julie B.

AU - Burgstaller, Jessica

AU - Laprell, Laura

AU - Konrad, David B.

AU - De La Osa de La Rosa, Luis

AU - Weaver, C. David

AU - Baier, Herwig

AU - Trauner, Dirk

AU - Barber, David M.

N1 - Publisher Copyright: © The Royal Society of Chemistry.

PY - 2017

Y1 - 2017

N2 - G-protein coupled inwardly rectifying potassium (GIRK) channels are an integral part of inhibitory signal transduction pathways, reducing the activity of excitable cells via hyperpolarization. They play crucial roles in processes such as cardiac output, cognition and the coordination of movement. Therefore, the precision control of GIRK channels is of critical importance. Here, we describe the development of the azobenzene containing molecule VLOGO (Visible Light Operated GIRK channel Opener), which activates GIRK channels in the dark and is promptly deactivated when illuminated with green light. VLOGO is a valuable addition to the existing tools for the optical control of GIRK channels as it circumvents the need to use potentially harmful UV irradiation. We therefore believe that VLOGO will be a useful research tool for studying GIRK channels in biological systems.

AB - G-protein coupled inwardly rectifying potassium (GIRK) channels are an integral part of inhibitory signal transduction pathways, reducing the activity of excitable cells via hyperpolarization. They play crucial roles in processes such as cardiac output, cognition and the coordination of movement. Therefore, the precision control of GIRK channels is of critical importance. Here, we describe the development of the azobenzene containing molecule VLOGO (Visible Light Operated GIRK channel Opener), which activates GIRK channels in the dark and is promptly deactivated when illuminated with green light. VLOGO is a valuable addition to the existing tools for the optical control of GIRK channels as it circumvents the need to use potentially harmful UV irradiation. We therefore believe that VLOGO will be a useful research tool for studying GIRK channels in biological systems.

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EP - 81

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 1

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Optical control of GIRK channels using visible light

Abstract

ASJC Scopus subject areas

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