Optical mapping of BAC clones from the human Y chromosome DAZ locus

Joseph Giacalone; Stephanie Delobette; Veronica Gibaja; Lei Ni; Yiannis Skiadas; Rong Qi; Joanne Edington; Zhongwu Lai; Damara Gebauer; Hongjuan Zhao; Thomas Anantharaman; Bhubaneswar Mishra; Laura G. Brown; Richa Saxena; David C. Page; David C. Schwartz

doi:10.1101/gr.112100

Optical mapping of BAC clones from the human Y chromosome DAZ locus

Joseph Giacalone, Stephanie Delobette, Veronica Gibaja, Lei Ni, Yiannis Skiadas, Rong Qi, Joanne Edington, Zhongwu Lai, Damara Gebauer, Hongjuan Zhao, Thomas Anantharaman, Bhubaneswar Mishra, Laura G. Brown, Richa Saxena, David C. Page, David C. Schwartz

Computer Science

Research output: Contribution to journal › Article › peer-review

Abstract

The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequences. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs representing four distinct copies of the DAZ gene and highlights a number of differences between individual copies of DAZ.

Original language	English (US)
Pages (from-to)	1421-1429
Number of pages	9
Journal	Genome Research
Volume	10
Issue number	9
DOIs	https://doi.org/10.1101/gr.112100
State	Published - 2000

ASJC Scopus subject areas

Genetics
Genetics(clinical)

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abstract = "The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequences. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs representing four distinct copies of the DAZ gene and highlights a number of differences between individual copies of DAZ.",

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AU - Giacalone, Joseph

AU - Delobette, Stephanie

AU - Gibaja, Veronica

AU - Ni, Lei

AU - Skiadas, Yiannis

AU - Qi, Rong

AU - Edington, Joanne

AU - Lai, Zhongwu

AU - Gebauer, Damara

AU - Zhao, Hongjuan

AU - Anantharaman, Thomas

AU - Mishra, Bhubaneswar

AU - Brown, Laura G.

AU - Saxena, Richa

AU - Page, David C.

AU - Schwartz, David C.

PY - 2000

Y1 - 2000

N2 - The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequences. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs representing four distinct copies of the DAZ gene and highlights a number of differences between individual copies of DAZ.

AB - The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequences. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs representing four distinct copies of the DAZ gene and highlights a number of differences between individual copies of DAZ.

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Optical mapping of BAC clones from the human Y chromosome DAZ locus

Abstract

ASJC Scopus subject areas

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