Optical mapping of BAC clones from the human Y chromosome DAZ locus

Joseph Giacalone, Stephanie Delobette, Veronica Gibaja, Lei Ni, Yiannis Skiadas, Rong Qi, Joanne Edington, Zhongwu Lai, Damara Gebauer, Hongjuan Zhao, Thomas Anantharaman, Bhubaneswar Mishra, Laura G. Brown, Richa Saxena, David C. Page, David C. Schwartz

Research output: Contribution to journalArticle

Abstract

The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequences. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs representing four distinct copies of the DAZ gene and highlights a number of differences between individual copies of DAZ.

Original languageEnglish (US)
Pages (from-to)1421-1429
Number of pages9
JournalGenome Research
Volume10
Issue number9
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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