Optical properties by time-resolved fluorescence Monte Carlo simulation

P. Kadimcherla; M. Sadoqi; S. Kumar

doi:10.1117/12.478178

Optical properties by time-resolved fluorescence Monte Carlo simulation

P. Kadimcherla, M. Sadoqi, S. Kumar

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this paper we describe a Monte Carlo simulation for time resolved fluorescence. In the past information on steady state measurements have been reported. However we feel that a lot more information and insight could be gained by the use of time resolved fluorescence spectroscopy. We have developed a Monte Carlo simulation to study the fluorescence signal generated by fluorophores distributed in a scattering medium. The simulation uses a semi-infinite medium with a thickness of 1cm. We have used the simulation to study the effect of the change in optical properties of the medium on the TPSF (temporal point spread function) generated. We have also investigated the effect of the increased radial separation of the detector on the TPSF. We have observed a shift in the Tmax (time at which the peak intensity is reached) in accordance with diffusion theory. We wanted to validate our simulation by seeing how well we could derive the optical properties of the medium from the TPSF produced from simulation. We fitted the TPSF to an adjusted form of the diffusion theory to find scattering coefficient, μ_s, and we have used an analytical model of time resolved fluorescence to extract the absorption coefficient, μ_a. The results obtained were better than previously reported.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	B. Chance, R.R. Alfano, B.J. Tromberg, M. Tamura, E.M. Sevick-Muraca
Pages	226-235
Number of pages	10
Volume	4955
DOIs	https://doi.org/10.1117/12.478178
State	Published - 2003
Event	PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Tomography and Spectroscopy of Tissue V - San Jose, CA, United States Duration: Jan 26 2003 → Jan 29 2003

Other

Other	PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Tomography and Spectroscopy of Tissue V
Country/Territory	United States
City	San Jose, CA
Period	1/26/03 → 1/29/03

Keywords

Absorption coefficient
Diffusion theory
Fluorescence
Monte Carlo
Optical properties
Scattering coefficient

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

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10.1117/12.478178

Cite this

Optical properties by time-resolved fluorescence Monte Carlo simulation. / Kadimcherla, P.; Sadoqi, M.; Kumar, S.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / B. Chance; R.R. Alfano; B.J. Tromberg; M. Tamura; E.M. Sevick-Muraca. Vol. 4955 2003. p. 226-235.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kadimcherla, P, Sadoqi, M & Kumar, S 2003, Optical properties by time-resolved fluorescence Monte Carlo simulation. in B Chance, RR Alfano, BJ Tromberg, M Tamura & EM Sevick-Muraca (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4955, pp. 226-235, PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Tomography and Spectroscopy of Tissue V, San Jose, CA, United States, 1/26/03. https://doi.org/10.1117/12.478178

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AB - In this paper we describe a Monte Carlo simulation for time resolved fluorescence. In the past information on steady state measurements have been reported. However we feel that a lot more information and insight could be gained by the use of time resolved fluorescence spectroscopy. We have developed a Monte Carlo simulation to study the fluorescence signal generated by fluorophores distributed in a scattering medium. The simulation uses a semi-infinite medium with a thickness of 1cm. We have used the simulation to study the effect of the change in optical properties of the medium on the TPSF (temporal point spread function) generated. We have also investigated the effect of the increased radial separation of the detector on the TPSF. We have observed a shift in the Tmax (time at which the peak intensity is reached) in accordance with diffusion theory. We wanted to validate our simulation by seeing how well we could derive the optical properties of the medium from the TPSF produced from simulation. We fitted the TPSF to an adjusted form of the diffusion theory to find scattering coefficient, μs, and we have used an analytical model of time resolved fluorescence to extract the absorption coefficient, μa. The results obtained were better than previously reported.

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KW - Monte Carlo

KW - Optical properties

KW - Scattering coefficient

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Optical properties by time-resolved fluorescence Monte Carlo simulation

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