Optical tomographic monitoring of vascular responses to anti-angiogenic drugs in preclinical tumor models

Molly L. Flexman, Hyun K. Kim, Sonia L. Hernandez, Jianzhong Huang, Tessa J. Johung, Jonghwan Lee, Darrell J. Yamashiro, Jessica J. Kandel, Andreas H. Hielscher

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    It is well acknowledged that treatment efficacy could be increased and unnecessary toxicities reduced if a rapid assessment strategy were available to allow individual tailoring of cancer therapy. In this work we focus on using optical tomographic imaging to detect tumor response to anti-angiogenic treatment within the first 5 days of therapy. For this study we used two models with well-characterized and divergent responses to inhibition of vascular endothelial growth factor (VEGF). SK-NEP and NGP cells were implanted intrarenally into NCR nude mice and the resulting tumors were monitored until a threshold of 1-2 g was reached. Optical tomographic imaging with a dual-wavelength (λ = 765nm and 830nm) continuous wave system, was performed prior to the first treatment with the anti-VEGF bevacizumab (BV), as well as 1, 3, and 5 days later. We found that the SK-NEP tumor model, known to be responsive to BV treatment, shows a decrease in hemoglobin levels over the 5 days. Mice implanted with the NGP tumor model, known to be less responsive to treatment, do not show such decreases. These results were further validated with histopathological findings that showed a decrease in tumor vascularization in treated SK-NEP mice. These results suggest that optical tomography is a promising tool for monitoring early tumor response to drug therapy.

    Original languageEnglish (US)
    Title of host publicationOptical Tomography and Spectroscopy of Tissue IX
    DOIs
    StatePublished - 2011
    EventOptical Tomography and Spectroscopy of Tissue IX - San Francisco, CA, United States
    Duration: Jan 23 2011Jan 26 2011

    Publication series

    NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
    Volume7896
    ISSN (Print)1605-7422

    Conference

    ConferenceOptical Tomography and Spectroscopy of Tissue IX
    CountryUnited States
    CitySan Francisco, CA
    Period1/23/111/26/11

    Keywords

    • angiogenesis
    • anti-angiogenic therapy
    • bevacizumab
    • cancer
    • Optical tomography
    • small animal imaging

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Atomic and Molecular Physics, and Optics
    • Biomaterials
    • Radiology Nuclear Medicine and imaging

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