Optoacoustic Tissue Differentiation Using a Mach-Zehnder Interferometer: Preliminary Results

Herve Nguendon Kenhagho, Georg Rauter, Raphael Guzman, Philippe Cattin, Azhar Zam

Research output: Chapter in Book/Report/Conference proceedingConference contribution


As laser osteotomy offers precise and small cuts with less trauma compared to conventional mechanical bone surgery. To fully exploit the advantages of laserosteotomes over conventional osteotomes, real-time feedback to differentiate hard bone from surrounding soft tissues is desired. In this study, we differentiated various tissue types - hard bone, fat and muscle tissue from an upper and lower fresh porcine thigh - based on cutting sounds. For laser ablation, an Nd:YAG laser was used to create ten craters on the surface of each upper and lower thigh. For sound recording, the probing beam of a Mach-Zehnder interferometer was placed 5cm away from each ablation site. For offline tissue differentiation, we investigated the measurements by looking at the amplitude of the spectrum. Then, we used Principle Component Analysis (PCA) to reduce the dimensionally and the 95% confidence ellipsoid (Mahalanobis distance) method to differentiate between tissues based on the acoustic shock wave. A set of 2520 data points, measured from the first seven craters of the upper and lower thigh, was used as 'training data' a set of 1080 data points from the last three craters was considered as 'testing data'. As seen in the confusion matrix, the experimental data from hard bone, fat and muscle yielded error rates of 0.46%, 0.19% and 1.30%, respectively. Preliminary results of this study demonstrate a promising technique for differentiating bone, fat and muscle tissues during laser surgery.

Original languageEnglish (US)
Title of host publication2018 IEEE International Ultrasonics Symposium, IUS 2018
PublisherIEEE Computer Society
ISBN (Electronic)9781538634257
StatePublished - Dec 17 2018
Event2018 IEEE International Ultrasonics Symposium, IUS 2018 - Kobe, Japan
Duration: Oct 22 2018Oct 25 2018

Publication series

NameIEEE International Ultrasonics Symposium, IUS
ISSN (Print)1948-5719
ISSN (Electronic)1948-5727


Conference2018 IEEE International Ultrasonics Symposium, IUS 2018


  • Acoustic tissue response
  • laser ablation
  • Mach-Zehnder interferometer
  • tissue differentiation

ASJC Scopus subject areas

  • Acoustics and Ultrasonics


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