Osmotic regulation of the mitochondrial permeability transition pore investigated by light scattering, fluorescence and electron microscopy techniques

Artyom Y. Baev, Pia A. Elustondo, Alexander Negoda, Evgeny V. Pavlov

Research output: Contribution to journalArticlepeer-review

Abstract

Mitochondrial permeability transition (PT) is a phenomenon of an increase of the inner membrane permeability in response to an excessive matrix calcium accumulation. PTP is caused by the opening of the large weakly selective channel. Molecular composition and regulation of permeability transition pore (PTP) are not well understood. Here we used isolated mitochondria to investigate dependence of PTP activation on the osmotic pressure. We found that in low osmotic strength solution calcium-induced PTP is significantly inhibited. We propose that this effect is linked to the changes in the curvature of the mitochondrial inner membrane. This interpretation is consistent with the idea about the importance of ATP synthase dimerization in modulation of the PTP activity.

Original languageEnglish (US)
Pages (from-to)38-44
Number of pages7
JournalAnalytical Biochemistry
Volume552
DOIs
StatePublished - Jul 1 2018

Keywords

  • ATP-synthase
  • C-subunit
  • Calcium
  • Mitochondrial permeability transition pore
  • Osmotic pressure
  • Swelling

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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