Overexpression of Bcl-2 suppresses the calcium activation of a mitochondrial megachannel

Robert C. Murphy, Erasmus Schneider, Kathleen W. Kinnally

    Research output: Contribution to journalArticlepeer-review


    The molecular mechanism(s) by which Bcl-2 regulates apoptosis is poorly understood. Bcl-2 suppresses apoptosis by inhibiting calcium activation of the permeability transition of mitochondria. In this patch-clamp study, overexpression of Bcl-2 in mitochondria of cultured cells suppressed calcium activation of a high conductance channel that may underlie the permeability transition. All other single channel parameters were identical when multiple conductance channel activities of mitochondria from control and Bcl-2 overexpressing cells were compared. Bcl-2 forms channels in artificial membranes; however, no novel channel activities could be linked to Bcl-2 overexpression, suggesting Bcl-2 does not form channels in native inner membranes of mitochondria.

    Original languageEnglish (US)
    Pages (from-to)73-76
    Number of pages4
    JournalFEBS Letters
    Issue number2-3
    StatePublished - May 25 2001


    • Apoptosis
    • Bcl-2
    • Channel
    • Mitochondrion
    • Patch clamp

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology


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