Paracrine regulation of neural crest EMT by placodal MMP28

Nadège Gouignard; Anne Bibonne; João F. Mata; Fernanda Bajanca; Bianka Berki; Elias H. Barriga; Jean Pierre Saint-Jeannet; Eric Theveneau

doi:10.1371/journal.pbio.3002261

Paracrine regulation of neural crest EMT by placodal MMP28

Nadège Gouignard, Anne Bibonne, João F. Mata, Fernanda Bajanca, Bianka Berki, Elias H. Barriga, Jean Pierre Saint-Jeannet, Eric Theveneau

Molecular Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

Epithelial–mesenchymal transition (EMT) is an early event in cell dissemination from epithelial tissues. EMT endows cells with migratory, and sometimes invasive, capabilities and is thus a key process in embryo morphogenesis and cancer progression. So far, matrix metalloproteinases (MMPs) have not been considered as key players in EMT but rather studied for their role in matrix remodelling in later events such as cell migration per se. Here, we used Xenopus neural crest (NC) cells to assess the role of MMP28 in EMT and migration in vivo. We show that a catalytically active MMP28, expressed by neighbouring placodal cells, is required for NC EMT and cell migration. We provide strong evidence indicating that MMP28 is imported in the nucleus of NC cells where it is required for normal Twist expression. Our data demonstrate that MMP28 can act as an upstream regulator of EMT in vivo raising the possibility that other MMPs might have similar early roles in various EMT-related contexts such as cancer, fibrosis, and wound healing.

Original language	English (US)
Article number	e3002261
Journal	PLoS biology
Volume	21
Issue number	8 August
DOIs	https://doi.org/10.1371/journal.pbio.3002261
State	Published - Aug 2023

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Agricultural and Biological Sciences(all)

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10.1371/journal.pbio.3002261

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title = "Paracrine regulation of neural crest EMT by placodal MMP28",

abstract = "Epithelial–mesenchymal transition (EMT) is an early event in cell dissemination from epithelial tissues. EMT endows cells with migratory, and sometimes invasive, capabilities and is thus a key process in embryo morphogenesis and cancer progression. So far, matrix metalloproteinases (MMPs) have not been considered as key players in EMT but rather studied for their role in matrix remodelling in later events such as cell migration per se. Here, we used Xenopus neural crest (NC) cells to assess the role of MMP28 in EMT and migration in vivo. We show that a catalytically active MMP28, expressed by neighbouring placodal cells, is required for NC EMT and cell migration. We provide strong evidence indicating that MMP28 is imported in the nucleus of NC cells where it is required for normal Twist expression. Our data demonstrate that MMP28 can act as an upstream regulator of EMT in vivo raising the possibility that other MMPs might have similar early roles in various EMT-related contexts such as cancer, fibrosis, and wound healing.",

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AU - Gouignard, Nadège

AU - Bibonne, Anne

AU - Mata, João F.

AU - Bajanca, Fernanda

AU - Berki, Bianka

AU - Barriga, Elias H.

AU - Saint-Jeannet, Jean Pierre

AU - Theveneau, Eric

N1 - Publisher Copyright: © 2023 Gouignard et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2023/8

Y1 - 2023/8

N2 - Epithelial–mesenchymal transition (EMT) is an early event in cell dissemination from epithelial tissues. EMT endows cells with migratory, and sometimes invasive, capabilities and is thus a key process in embryo morphogenesis and cancer progression. So far, matrix metalloproteinases (MMPs) have not been considered as key players in EMT but rather studied for their role in matrix remodelling in later events such as cell migration per se. Here, we used Xenopus neural crest (NC) cells to assess the role of MMP28 in EMT and migration in vivo. We show that a catalytically active MMP28, expressed by neighbouring placodal cells, is required for NC EMT and cell migration. We provide strong evidence indicating that MMP28 is imported in the nucleus of NC cells where it is required for normal Twist expression. Our data demonstrate that MMP28 can act as an upstream regulator of EMT in vivo raising the possibility that other MMPs might have similar early roles in various EMT-related contexts such as cancer, fibrosis, and wound healing.

AB - Epithelial–mesenchymal transition (EMT) is an early event in cell dissemination from epithelial tissues. EMT endows cells with migratory, and sometimes invasive, capabilities and is thus a key process in embryo morphogenesis and cancer progression. So far, matrix metalloproteinases (MMPs) have not been considered as key players in EMT but rather studied for their role in matrix remodelling in later events such as cell migration per se. Here, we used Xenopus neural crest (NC) cells to assess the role of MMP28 in EMT and migration in vivo. We show that a catalytically active MMP28, expressed by neighbouring placodal cells, is required for NC EMT and cell migration. We provide strong evidence indicating that MMP28 is imported in the nucleus of NC cells where it is required for normal Twist expression. Our data demonstrate that MMP28 can act as an upstream regulator of EMT in vivo raising the possibility that other MMPs might have similar early roles in various EMT-related contexts such as cancer, fibrosis, and wound healing.

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Paracrine regulation of neural crest EMT by placodal MMP28

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