Parathyroid hormone induces the nuclear orphan receptor NOR-1 in osteoblasts

Flavia Q. Pirih, Jeanne M. Nervina, Lee Pham, Tara Aghaloo, Sotirios Tetradis

Research output: Contribution to journalArticlepeer-review

Abstract

Parathyroid hormone (PTH) significantly affects osteoblast function by altering gene expression. We have identified neuron-derived orphan receptor-1 (NOR-1) as a PTH-induced primary gene in osteoblastic cells. NOR-1, Nurr1, and Nur77 comprise the NGFI-B nuclear orphan receptor family and Nurr1 and Nur77 are PTH-induced primary osteoblastic genes. Ten nM PTH maximally induced NOR-1 mRNA at 2h in primary mouse osteoblasts and at 1h in mouse calvariae. Cycloheximide pretreatment did not inhibit PTH-induced NOR-1 mRNA. PTH activates cAMP-protein kinase A (PKA), protein kinase C (PKC), and calcium signaling. Forskolin (PKA activator) and PMA (PKC activator) mimicked PTH-induced NOR-1 mRNA. Ionomycin (calcium ionophore) and PTH(3-34), which do not activate PKA, failed to induce NOR-1 mRNA. PKA inhibition with H89 blocked PTH- and FSK-induced NOR-1 mRNA. PMA pretreatment to deplete PKC inhibited PMA-induced, but not PTH-induced, NOR-1 mRNA. We conclude that NOR-1 is a PTH-regulated primary osteoblastic gene that is induced mainly through cAMP-PKA signaling.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume306
Issue number1
DOIs
StatePublished - Jun 20 2003

Keywords

  • NGFI-B
  • NOR-1
  • Orphan nuclear receptor
  • Osteoblast
  • PTH

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Parathyroid hormone induces the nuclear orphan receptor NOR-1 in osteoblasts'. Together they form a unique fingerprint.

Cite this