Abstract
Human submandibular/sublingual saliva contains a protein that promotes adhesion of Streptococcus mutans JBP serotype-c to spheroidal hydroxyapatite in vitro. A high molecular-weight (250,000-300,000 Da) adhesion-promoting protein (APP) was purified by Trisacryl 2000 M gel-filtration chromatography and gel electroelution before it was partially characterized. Lectin blotting identified that the terminal carbohydrates include N-acetyl glucosamine-β]-4-N-acetylglucosamine, galactose and galactose-β]-3-N-acetyl galactosamine. Antibodies to APP demonstrated no difference in the immunoreactive pattern of APP from saliva of caries-active or caries-resistant individuals belonging to four different ethnic groups: Asian, African-American, Hispanic or Caucasian. No immunological similarities to salivary mucins or parotid agglutinins were detected by Western blotting using immuno-cross- reactivity as a criterion. APP appears to be a unique protein found in submandibular/sublingual saliva. Understanding such a protein could help prevent S. mutans attachment to the enamel surface.
Original language | English (US) |
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Pages (from-to) | 337-345 |
Number of pages | 9 |
Journal | Archives of Oral Biology |
Volume | 47 |
Issue number | 5 |
DOIs | |
State | Published - 2002 |
Keywords
- Antibodies
- Bacterial adhesion
- Chromatography
- Dental caries
- Hydroxyapatite
- Immunoglobulins
- Lectins
- Saliva
- Streptococcus mutans
ASJC Scopus subject areas
- Otorhinolaryngology
- Dentistry(all)
- Cell Biology