Partial characterization of a human submandibular/sublingual salivary adhesion-promoting protein

S. O. Akintoye, M. Dasso, D. I. Hay, N. Ganeshkumar, A. I. Spielman

Research output: Contribution to journalArticlepeer-review


Human submandibular/sublingual saliva contains a protein that promotes adhesion of Streptococcus mutans JBP serotype-c to spheroidal hydroxyapatite in vitro. A high molecular-weight (250,000-300,000 Da) adhesion-promoting protein (APP) was purified by Trisacryl 2000 M gel-filtration chromatography and gel electroelution before it was partially characterized. Lectin blotting identified that the terminal carbohydrates include N-acetyl glucosamine-β]-4-N-acetylglucosamine, galactose and galactose-β]-3-N-acetyl galactosamine. Antibodies to APP demonstrated no difference in the immunoreactive pattern of APP from saliva of caries-active or caries-resistant individuals belonging to four different ethnic groups: Asian, African-American, Hispanic or Caucasian. No immunological similarities to salivary mucins or parotid agglutinins were detected by Western blotting using immuno-cross- reactivity as a criterion. APP appears to be a unique protein found in submandibular/sublingual saliva. Understanding such a protein could help prevent S. mutans attachment to the enamel surface.

Original languageEnglish (US)
Pages (from-to)337-345
Number of pages9
JournalArchives of Oral Biology
Issue number5
StatePublished - 2002


  • Antibodies
  • Bacterial adhesion
  • Chromatography
  • Dental caries
  • Hydroxyapatite
  • Immunoglobulins
  • Lectins
  • Saliva
  • Streptococcus mutans

ASJC Scopus subject areas

  • Otorhinolaryngology
  • General Dentistry
  • Cell Biology


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