Partial purification of a novel stimulant of gastric acid secretion from canine non-antral mucosa

N. W. Bunnett; M. S. Orloff; Y. Coto; H. J. Corbet; R. Garcia; J. R. Reeve; H. T. Debas; J. H. Walsh

doi:10.1016/0024-3205(86)90256-0

Partial purification of a novel stimulant of gastric acid secretion from canine non-antral mucosa

N. W. Bunnett, M. S. Orloff, Y. Coto, H. J. Corbet, R. Garcia, J. R. Reeve, H. T. Debas, J. H. Walsh

Molecular Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

A novel stimulant of gastric acid secretion was extracted and purified from the non-antral gastric mucosa of the canine stomach and some of its biological properties were examined. Tissue was boiled in water and extracted in 2% trifluoroacetic acid. The stimulatory activity was purified by a combination of reverse-phase high pressure liquid chromatography (HPLC) and gel filtration. Fractions were assayed for a stimulation of basal, pentagastrin-and histamine-stimulated gastric acid secretion in the anaesthetized rat. Stimulatory activity was eluted from reversephase HPLC columns with acetonitrile and its elution from Sephadex G-10 and G-50 columns suggested a molecular weight of 1,000 to 3,000. The highly purified extracts enhanced basal, pentagastrin-and histamine-stimulated acid secretion in the rat. A stimulatory fraction was purified which was devoid of immunoreactive gastrin and gastrin-releasing peptide and contained only small amounts of histamine. Its chromatographic properties differed from those of histamine and acetycholine. On two occasions the stimulant was purified to homogeneity and found to contain amino acids. Insufficient pure material was obtained for full characterization. The stimulant has been tentatively called oxyntin.

Original language	English (US)
Pages (from-to)	887-894
Number of pages	8
Journal	Life Sciences
Volume	38
Issue number	10
DOIs	https://doi.org/10.1016/0024-3205(86)90256-0
State	Published - Mar 10 1986

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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title = "Partial purification of a novel stimulant of gastric acid secretion from canine non-antral mucosa",

abstract = "A novel stimulant of gastric acid secretion was extracted and purified from the non-antral gastric mucosa of the canine stomach and some of its biological properties were examined. Tissue was boiled in water and extracted in 2% trifluoroacetic acid. The stimulatory activity was purified by a combination of reverse-phase high pressure liquid chromatography (HPLC) and gel filtration. Fractions were assayed for a stimulation of basal, pentagastrin-and histamine-stimulated gastric acid secretion in the anaesthetized rat. Stimulatory activity was eluted from reversephase HPLC columns with acetonitrile and its elution from Sephadex G-10 and G-50 columns suggested a molecular weight of 1,000 to 3,000. The highly purified extracts enhanced basal, pentagastrin-and histamine-stimulated acid secretion in the rat. A stimulatory fraction was purified which was devoid of immunoreactive gastrin and gastrin-releasing peptide and contained only small amounts of histamine. Its chromatographic properties differed from those of histamine and acetycholine. On two occasions the stimulant was purified to homogeneity and found to contain amino acids. Insufficient pure material was obtained for full characterization. The stimulant has been tentatively called oxyntin.",

author = "Bunnett, {N. W.} and Orloff, {M. S.} and Y. Coto and Corbet, {H. J.} and R. Garcia and Reeve, {J. R.} and Debas, {H. T.} and Walsh, {J. H.}",

note = "Funding Information: Supported by a NATO exchange grant 937/83, the Medical Research Council (UK) and NIH grants. Dorothea Aures-Fischer and Tom Kovacs measured histamine, gastrin and gastrin-releasing peptides.",

year = "1986",

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doi = "10.1016/0024-3205(86)90256-0",

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T1 - Partial purification of a novel stimulant of gastric acid secretion from canine non-antral mucosa

AU - Bunnett, N. W.

AU - Orloff, M. S.

AU - Coto, Y.

AU - Corbet, H. J.

AU - Garcia, R.

AU - Reeve, J. R.

AU - Debas, H. T.

AU - Walsh, J. H.

N1 - Funding Information: Supported by a NATO exchange grant 937/83, the Medical Research Council (UK) and NIH grants. Dorothea Aures-Fischer and Tom Kovacs measured histamine, gastrin and gastrin-releasing peptides.

PY - 1986/3/10

Y1 - 1986/3/10

N2 - A novel stimulant of gastric acid secretion was extracted and purified from the non-antral gastric mucosa of the canine stomach and some of its biological properties were examined. Tissue was boiled in water and extracted in 2% trifluoroacetic acid. The stimulatory activity was purified by a combination of reverse-phase high pressure liquid chromatography (HPLC) and gel filtration. Fractions were assayed for a stimulation of basal, pentagastrin-and histamine-stimulated gastric acid secretion in the anaesthetized rat. Stimulatory activity was eluted from reversephase HPLC columns with acetonitrile and its elution from Sephadex G-10 and G-50 columns suggested a molecular weight of 1,000 to 3,000. The highly purified extracts enhanced basal, pentagastrin-and histamine-stimulated acid secretion in the rat. A stimulatory fraction was purified which was devoid of immunoreactive gastrin and gastrin-releasing peptide and contained only small amounts of histamine. Its chromatographic properties differed from those of histamine and acetycholine. On two occasions the stimulant was purified to homogeneity and found to contain amino acids. Insufficient pure material was obtained for full characterization. The stimulant has been tentatively called oxyntin.

AB - A novel stimulant of gastric acid secretion was extracted and purified from the non-antral gastric mucosa of the canine stomach and some of its biological properties were examined. Tissue was boiled in water and extracted in 2% trifluoroacetic acid. The stimulatory activity was purified by a combination of reverse-phase high pressure liquid chromatography (HPLC) and gel filtration. Fractions were assayed for a stimulation of basal, pentagastrin-and histamine-stimulated gastric acid secretion in the anaesthetized rat. Stimulatory activity was eluted from reversephase HPLC columns with acetonitrile and its elution from Sephadex G-10 and G-50 columns suggested a molecular weight of 1,000 to 3,000. The highly purified extracts enhanced basal, pentagastrin-and histamine-stimulated acid secretion in the rat. A stimulatory fraction was purified which was devoid of immunoreactive gastrin and gastrin-releasing peptide and contained only small amounts of histamine. Its chromatographic properties differed from those of histamine and acetycholine. On two occasions the stimulant was purified to homogeneity and found to contain amino acids. Insufficient pure material was obtained for full characterization. The stimulant has been tentatively called oxyntin.

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Partial purification of a novel stimulant of gastric acid secretion from canine non-antral mucosa

Abstract

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