Partial purification of a novel stimulant of gastric acid secretion from canine non-antral mucosa

N. W. Bunnett, M. S. Orloff, Y. Coto, H. J. Corbet, R. Garcia, J. R. Reeve, H. T. Debas, J. H. Walsh

Research output: Contribution to journalArticle

Abstract

A novel stimulant of gastric acid secretion was extracted and purified from the non-antral gastric mucosa of the canine stomach and some of its biological properties were examined. Tissue was boiled in water and extracted in 2% trifluoroacetic acid. The stimulatory activity was purified by a combination of reverse-phase high pressure liquid chromatography (HPLC) and gel filtration. Fractions were assayed for a stimulation of basal, pentagastrin-and histamine-stimulated gastric acid secretion in the anaesthetized rat. Stimulatory activity was eluted from reversephase HPLC columns with acetonitrile and its elution from Sephadex G-10 and G-50 columns suggested a molecular weight of 1,000 to 3,000. The highly purified extracts enhanced basal, pentagastrin-and histamine-stimulated acid secretion in the rat. A stimulatory fraction was purified which was devoid of immunoreactive gastrin and gastrin-releasing peptide and contained only small amounts of histamine. Its chromatographic properties differed from those of histamine and acetycholine. On two occasions the stimulant was purified to homogeneity and found to contain amino acids. Insufficient pure material was obtained for full characterization. The stimulant has been tentatively called oxyntin.

Original languageEnglish (US)
Pages (from-to)887-894
Number of pages8
JournalLife Sciences
Volume38
Issue number10
DOIs
StatePublished - Mar 10 1986

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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