Particle-Based Microrheology As a Tool for Characterizing Protein-Based Materials

Michael Meleties, Rhett L. Martineau, Maneesh K. Gupta, Jin Kim Montclare

Research output: Contribution to journalReview articlepeer-review


Microrheology based on video microscopy of embedded tracer particles has the potential to be used for high-throughput protein-based materials characterization. This potential is due to a number of characteristics of the techniques, including the suitability for measurement of low sample volumes, noninvasive and noncontact measurements, and the ability to set up a large number of samples for facile, sequential measurement. In addition to characterization of the bulk rheological properties of proteins in solution, for example, viscosity, microrheology can provide insight into the dynamics and self-assembly of protein-based materials as well as heterogeneities in the microenvironment being probed. Specifically, passive microrheology in the form of multiple particle tracking and differential dynamic microscopy holds promise for applications in high-throughput characterization because of the lack of user interaction required while making measurements. Herein, recent developments in the use of multiple particle tracking and differential dynamic microscopy are reviewed for protein characterization and their potential to be applied in a high-throughput, automatable setting.

Original languageEnglish (US)
Pages (from-to)2747-2763
Number of pages17
JournalACS Biomaterials Science and Engineering
Issue number7
StatePublished - Jul 11 2022


  • differential dynamic microscopy
  • microrheology
  • multiple particle tracking
  • protein biomaterials
  • self-assembly
  • Proteins
  • Rheology/methods
  • Viscosity

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials


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