"Perfect" designer chromosome v and behavior of a ring derivative

Ze Xiong Xie, Bing Zhi Li, Leslie A. Mitchell, Yi Wu, Xin Qi, Zhu Jin, Bin Jia, Xia Wang, Bo Xuan Zeng, Hui Min Liu, Xiao Le Wu, Qi Feng, Wen Zheng Zhang, Wei Liu, Ming Zhu Ding, Xia Li, Guang Rong Zhao, Jian Jun Qiao, Jing Sheng Cheng, Meng ZhaoZheng Kuang, Xuya Wang, J. Andrew Martin, Giovanni Stracquadanio, Kun Yang, Xue Bai, Juan Zhao, Meng Long Hu, Qiu Hui Lin, Wen Qian Zhang, Ming Hua Shen, Si Chen, Wan Su, En Xu Wang, Rui Guo, Fang Zhai, Xue Jiao Guo, Hao Xing Du, Jia Qing Zhu, Tian Qing Song, Jun Jun Dai, Fei Fei Li, Guo Zhen Jiang, Shi Lei Han, Shi Yang Liu, Zhi Chao Yu, Xiao Na Yang, Ken Chen, Cheng Hu, Da Shuai Li, Nan Jia, Yue Liu, Lin Ting Wang, Su Wang, Xiao Tong Wei, Mei Qing Fu, Lan Meng Qu, Si Yu Xin, Ting Liu, Kai Ren Tian, Xue Nan Li, Jin Hua Zhang, Li Xiang Song, Jin Gui Liu, Jia Fei Lv, Hang Xu, Ran Tao, Yan Wang, Ting Ting Zhang, Ye Xuan Deng, Yi Ran Wang, Ting Li, Guang Xin Ye, Xiao Ran Xu, Zheng Bao Xia, Wei Zhang, Shi Lan Yang, Yi Lin Liu, Wen Qi Ding, Zhen Ning Liu, Jun Qi Zhu, Ning Zhi Liu, Roy Walker, Yisha Luo, Yun Wang, Yue Shen, Huanming Yang, Yizhi Cai, Ping Sheng Ma, Chun Ting Zhang, Joel S. Bader, Jef D. Boeke, Ying Jin Yuan

    Research output: Contribution to journalArticle

    Abstract

    Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis.We designed and de novo synthesized 536, 024-base pair chromosome synV in the "Build-A-Genome China" course.We corrected an initial isolate of synV to perfectly match the designed sequence using integrative cotransformation and clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness under a variety of culture conditions, compared with that of wild-type V strains. A ring synV derivative was constructed, which is fully functional in Saccharomyces cerevisiae under all conditions tested and exhibits lower spore viability during meiosis. Ring synV chromosome can extends Sc2.0 design principles and provides a model with which to study genomic rearrangement, ring chromosome evolution, and human ring chromosome disorders.

    Original languageEnglish (US)
    Article numbereaaf4704
    JournalScience
    Volume355
    Issue number6329
    DOIs
    StatePublished - Mar 10 2017

    ASJC Scopus subject areas

    • General

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