TY - JOUR
T1 - Periodontal Tissue Regeneration Using Brain-Derived Neurotrophic Factor Delivered by Collagen Sponge
AU - Ramalho, Ilana
AU - Bergamo, Edmara
AU - Lopes, Adolfo
AU - Medina-Cintrón, Camille
AU - Neiva, Rodrigo
AU - Witek, Lukasz
AU - Coelho, Paulo
N1 - Funding Information:
The authors would like to thank the Dentsply Sirona (PI-PGC), for providing the funding to conduct this study, the Coordenac¸ão de Aperfeic¸oamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 (grant no. 88881.132824/2016-0 and 88881.132654/2016-01) and the São Paulo Research Foundation (FAPESP)(grant no. 2016/ 17793-1; 2017/19362-0), for providing scholarships to I.R., E.B. and A.L.
Publisher Copyright:
© Copyright 2019, Mary Ann Liebert, Inc., publishers 2019.
PY - 2019/8
Y1 - 2019/8
N2 - Aim: To evaluate the influence of brain-derived neurotrophic factor (BDNF) in combination with collagen sponges on periodontal tissue regeneration. Methods: Unilateral, "box-Type" (4 × 5 mm), one-wall intrabony defects were surgically created at posterior mandibular teeth in 14 Beagle dogs. Animals received all experimental groups, and the defects were randomly treated as follows: Emdogain® (positive control) [EMD]; HeliPlug®+BDNF [H/B]; RCP®+BDNF [R/B]; negative control [Control]; TeruPlug®+BDNF [Tp/B]; and TeruPlug®+BDNF2 [Ts/B]. Periodontal wound healing was observed every 2 weeks by computed tomography. The animals were euthanized at 8 weeks postsurgery for microcomputed tomography and histomorphometric evaluation. Results: All groups presented ∼1 mm apical epithelial attachment relative to cementoenamel junction. Although linear measurements did not demonstrate significant differences between groups for cementum and periodontal ligament regeneration, semiquantitative analysis depicted higher percentage of samples with mineralized cementum and functional PDL for Ts/B, R/B, and H/B groups relative to EMD and Control (p < 0.046). Irrespective of quantification method (2D or 3D), Ts/B, Control, Tp/B, and H/B groups presented the highest mean percentage of new bone (not significantly different) followed by R/B and EMD groups. Conclusion: While no significant differences were detected in quantitative analyses, Ts/B combination results in significantly more samples with full periodontal tissue regeneration relative to control groups. The various roles played by brain-derived neurotrophic factor (BDNF) in a multitude of tissues and at different scenarios have rendered BDNF a favorable candidate for improving tissue regeneration. Although the tested formulations of BDNF quantitatively regenerate tissue to a level similar to control groups, it resulted in significantly more instances of full regeneration.
AB - Aim: To evaluate the influence of brain-derived neurotrophic factor (BDNF) in combination with collagen sponges on periodontal tissue regeneration. Methods: Unilateral, "box-Type" (4 × 5 mm), one-wall intrabony defects were surgically created at posterior mandibular teeth in 14 Beagle dogs. Animals received all experimental groups, and the defects were randomly treated as follows: Emdogain® (positive control) [EMD]; HeliPlug®+BDNF [H/B]; RCP®+BDNF [R/B]; negative control [Control]; TeruPlug®+BDNF [Tp/B]; and TeruPlug®+BDNF2 [Ts/B]. Periodontal wound healing was observed every 2 weeks by computed tomography. The animals were euthanized at 8 weeks postsurgery for microcomputed tomography and histomorphometric evaluation. Results: All groups presented ∼1 mm apical epithelial attachment relative to cementoenamel junction. Although linear measurements did not demonstrate significant differences between groups for cementum and periodontal ligament regeneration, semiquantitative analysis depicted higher percentage of samples with mineralized cementum and functional PDL for Ts/B, R/B, and H/B groups relative to EMD and Control (p < 0.046). Irrespective of quantification method (2D or 3D), Ts/B, Control, Tp/B, and H/B groups presented the highest mean percentage of new bone (not significantly different) followed by R/B and EMD groups. Conclusion: While no significant differences were detected in quantitative analyses, Ts/B combination results in significantly more samples with full periodontal tissue regeneration relative to control groups. The various roles played by brain-derived neurotrophic factor (BDNF) in a multitude of tissues and at different scenarios have rendered BDNF a favorable candidate for improving tissue regeneration. Although the tested formulations of BDNF quantitatively regenerate tissue to a level similar to control groups, it resulted in significantly more instances of full regeneration.
KW - BDNF
KW - brain-derived neurotrophic factor
KW - collagen sponge
KW - emdogain
KW - periodontal regeneration
KW - Brain-Derived Neurotrophic Factor/pharmacology
KW - Dental Cementum/drug effects
KW - Epithelium/drug effects
KW - X-Ray Microtomography
KW - Collagen/pharmacology
KW - Animals
KW - Periodontium/diagnostic imaging
KW - Regeneration/physiology
KW - Bone Regeneration/drug effects
KW - Dogs
KW - Female
KW - Imaging, Three-Dimensional
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U2 - 10.1089/ten.tea.2018.0209
DO - 10.1089/ten.tea.2018.0209
M3 - Article
C2 - 30489221
AN - SCOPUS:85070454150
VL - 25
SP - 1072
EP - 1083
JO - Tissue Engineering - Part A.
JF - Tissue Engineering - Part A.
SN - 1937-3341
IS - 15-16
ER -