PFG-ω1-Filtered TOCSY Experiments for the Determination of Long-Range Heteronuclear and Homonuclear Coupling Constants and Estimation of J-Coupling "Crosstalk" Artifacts in 2-D ω1-Filtered "E. COSY-Style" Spectra

Guangzhao Xu, Bo Zhang, John Spencer Evans

Research output: Contribution to journalArticle

Abstract

We present novel one- and two-dimensional versions of the ω1-filtered TOCSY experiment. These experiments utilize pulsed-field gradient techniques and INEPT-reverse INEPT magnetization transfer to generate heteronuclear filtering by means of coherence pathway selection. The major advantages of this approach are twofold: first, each experiment requires a reasonable number of transmitter pulses, gradient pulses, and delays to implement. Second, the use of z-axis gradients at the beginning and termination of the pulse sequences prevents the recovery of dephased magnetization prior to FID detection. This technique was incorporated into 1-D and 2-D ω1-filtered JXH- and JHH-TOCSY-style experiments. As demonstrated on 15N-enriched peptide samples, the use of the pulsed-field-gradient coherence selection scheme effectively filters out unwanted magnetization components, thereby improving the overall sensitivity of the experiments. In addition to this suite of pulse sequences, we also present a method for correcting the reduction in J-coupling that results from crosspeak shifting in 2-D ω1-filtered E. COSY-style spectra. This correction is applicable to both Lorentzian and Gaussian 2-D crosspeak lineshapes.

Original languageEnglish (US)
Pages (from-to)127-134
Number of pages8
JournalJournal of Magnetic Resonance
Volume138
Issue number1
DOIs
StatePublished - May 1999

Keywords

  • Crosstalk J-coupling correction
  • Crosstalk relaxation
  • Heteronuclear
  • Homonuclear
  • Isotropic mixing
  • J-coupling
  • Peptides
  • Pulsed-field gradient

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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