TY - JOUR
T1 - Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling
AU - Thakur, Rajan
AU - Panda, Aniruddha
AU - Coessens, Elise
AU - Raj, Nikita
AU - Yadav, Shweta
AU - Balakrishnan, Sruthi
AU - Zhang, Qifeng
AU - Georgiev, Plamen
AU - Basak, Bishal
AU - Pasricha, Renu
AU - Wakelam, Michael J.O.
AU - Ktistakis, Nicholas T.
AU - Raghu, Padinjat
N1 - Funding Information:
Wellcome Trust DBT India Alliance IA/S/14/2/501540 Rajan Thakur Nikita Raj Shweta Yadav Sruthi Balakrishnan Bishal Basak Raghu Padinjat National Centre for Biological Sciences core Rajan Thakur Aniruddha Panda Nikita Raj Shweta Yadav Sruthi Balakrishnan Bishal Basak Renu Pasricha Raghu Padinjat Department of Biotechnology, BT/PR4833/MED/30/744/ Rajan Thakur Ministry of Science and Technology 2012 Aniruddha Panda Nikita Raj Shweta Yadav Sruthi Balakrishnan Bishal Basak Renu Pasricha Raghu Padinjat Biotechnology and Biological Sciences Research Council core Elise Coessens Qifeng Zhang Plamen Georgiev Michael JO Wakelam Nicholas T Ktistakis
PY - 2016/11/16
Y1 - 2016/11/16
N2 - During illumination, the light-sensitive plasma membrane (rhabdomere) of Drosophila photoreceptors undergoes turnover with consequent changes in size and composition. However, the mechanism by which illumination is coupled to rhabdomere turnover remains unclear. We find that photoreceptors contain a light-dependent phospholipase D (PLD) activity. During illumination, loss of PLD resulted in an enhanced reduction in rhabdomere size, accumulation of Rab7 positive, rhodopsin1-containing vesicles (RLVs) in the cell body and reduced rhodopsin protein. These phenotypes were associated with reduced levels of phosphatidic acid, the product of PLD activity and were rescued by reconstitution with catalytically active PLD. In wild-type photoreceptors, during illumination, enhanced PLD activity was sufficient to clear RLVs from the cell body by a process dependent on Arf1-GTP levels and retromer complex function. Thus, during illumination, PLD activity couples endocytosis of RLVs with their recycling to the plasma membrane thus maintaining plasma membrane size and composition.
AB - During illumination, the light-sensitive plasma membrane (rhabdomere) of Drosophila photoreceptors undergoes turnover with consequent changes in size and composition. However, the mechanism by which illumination is coupled to rhabdomere turnover remains unclear. We find that photoreceptors contain a light-dependent phospholipase D (PLD) activity. During illumination, loss of PLD resulted in an enhanced reduction in rhabdomere size, accumulation of Rab7 positive, rhodopsin1-containing vesicles (RLVs) in the cell body and reduced rhodopsin protein. These phenotypes were associated with reduced levels of phosphatidic acid, the product of PLD activity and were rescued by reconstitution with catalytically active PLD. In wild-type photoreceptors, during illumination, enhanced PLD activity was sufficient to clear RLVs from the cell body by a process dependent on Arf1-GTP levels and retromer complex function. Thus, during illumination, PLD activity couples endocytosis of RLVs with their recycling to the plasma membrane thus maintaining plasma membrane size and composition.
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U2 - 10.7554/eLife.18515
DO - 10.7554/eLife.18515
M3 - Article
C2 - 27848911
AN - SCOPUS:85001122898
VL - 5
JO - eLife
JF - eLife
SN - 2050-084X
IS - NOVEMBER2016
M1 - e18515
ER -