Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U

Maya Ameyar-Zazoua, Mouloud Souidi, Lauriane Fritsch, Philippe Robin, Audrey Thomas, Ali Hamiche, Piergiorgio Percipalle, Slimane Ait-Si-Ali, Annick Harel-Bellan

Research output: Contribution to journalArticle


By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1α (HP1α) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1α-interacting molecule. More importantly, hnRNP U depletion reduces HP1α-dependent gene silencing and disturbs HP1α subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1α function, shedding new light on the mode of action of HP1α and on the function of hnRNP U.

Original languageEnglish (US)
Pages (from-to)27974-27979
Number of pages6
JournalJournal of Biological Chemistry
Issue number41
StatePublished - Oct 9 2009


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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