TY - JOUR
T1 - Physical and functional interaction between heterochromatin protein 1α and the RNA-binding protein heterogeneous nuclear ribonucleoprotein U
AU - Ameyar-Zazoua, Maya
AU - Souidi, Mouloud
AU - Fritsch, Lauriane
AU - Robin, Philippe
AU - Thomas, Audrey
AU - Hamiche, Ali
AU - Percipalle, Piergiorgio
AU - Ait-Si-Ali, Slimane
AU - Harel-Bellan, Annick
PY - 2009/10/9
Y1 - 2009/10/9
N2 - By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1α (HP1α) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1α-interacting molecule. More importantly, hnRNP U depletion reduces HP1α-dependent gene silencing and disturbs HP1α subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1α function, shedding new light on the mode of action of HP1α and on the function of hnRNP U.
AB - By combining biochemical purification and mass spectrometry, we identified proteins associated with human heterochromatin protein 1α (HP1α) both in the nucleoplasm and in chromatin. Some of these are RNA-binding proteins, and among them is the protein heterogeneous nuclear ribonucleoprotein U (hnRNP U)/SAF-A, which is linked to chromatin organization and transcriptional regulation. Here, we demonstrate that hnRNP U is a bona fide HP1α-interacting molecule. More importantly, hnRNP U depletion reduces HP1α-dependent gene silencing and disturbs HP1α subcellular localization. Thus, our data demonstrate that hnRNP U is involved in HP1α function, shedding new light on the mode of action of HP1α and on the function of hnRNP U.
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U2 - 10.1074/jbc.M109.037929
DO - 10.1074/jbc.M109.037929
M3 - Article
C2 - 19617346
AN - SCOPUS:70350463532
SN - 0021-9258
VL - 284
SP - 27974
EP - 27979
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 41
ER -