TY - JOUR
T1 - Physico/chemical characterization and preliminary human histology assessment of a β-TCP particulate material for bone augmentation
AU - Coelho, Paulo G.
AU - Coimbra, Maria E.
AU - Ribeiro, Cristiane
AU - Fancio, Elizabeth
AU - Higa, Olga
AU - Suzuki, Marcelo
AU - Marincola, Mauro
PY - 2009/8/31
Y1 - 2009/8/31
N2 - This study aimed to physico/chemically characterize and evaluate the in vivo performance of a β-TCP particulate grafting material. SEM/TEM, and EDS and XPS were used for morphology and chemistry assessment, respectively. FTIR was used to determine Ca-P phases characteristic bands. Rietveld refinement/XRD spectra was performed for secondary phase detection. Particle size distribution and specific surface were assessed by a scattering-laser based technique and BET, respectively. Mercury porosimetry was employed to determine pore-size distribution. For in vivo evaluation, the grafting material was used in 12 patients' sinus lifts, and biopsies were obtained at post-operative times of 3, 6, and 9 months. SEM/TEM revealed multigrained particles with interconnected pores. EDS showed Ca, P, and O, with stoichiometry close to theoretical values. XRD/Rietveld showed that the material presented crystalline β-TCP with ~ 9% β-Ca2P2O7 secondary phase. FTIR did not detect the presence of bands related to α-TCP. Human histologic assessment showed that newly formed bone was present at 3 months, and degrees of bone organization increased as time elapsed in vivo. Human histology showed that the material is suitable for bone regeneration in a maxillofacial complex region.
AB - This study aimed to physico/chemically characterize and evaluate the in vivo performance of a β-TCP particulate grafting material. SEM/TEM, and EDS and XPS were used for morphology and chemistry assessment, respectively. FTIR was used to determine Ca-P phases characteristic bands. Rietveld refinement/XRD spectra was performed for secondary phase detection. Particle size distribution and specific surface were assessed by a scattering-laser based technique and BET, respectively. Mercury porosimetry was employed to determine pore-size distribution. For in vivo evaluation, the grafting material was used in 12 patients' sinus lifts, and biopsies were obtained at post-operative times of 3, 6, and 9 months. SEM/TEM revealed multigrained particles with interconnected pores. EDS showed Ca, P, and O, with stoichiometry close to theoretical values. XRD/Rietveld showed that the material presented crystalline β-TCP with ~ 9% β-Ca2P2O7 secondary phase. FTIR did not detect the presence of bands related to α-TCP. Human histologic assessment showed that newly formed bone was present at 3 months, and degrees of bone organization increased as time elapsed in vivo. Human histology showed that the material is suitable for bone regeneration in a maxillofacial complex region.
KW - Biocompatibility
KW - Human histology
KW - In vitro test
KW - Physicho/chemical characterization
KW - β-TCP
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UR - http://www.scopus.com/inward/citedby.url?scp=68149109973&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2009.04.013
DO - 10.1016/j.msec.2009.04.013
M3 - Article
AN - SCOPUS:68149109973
SN - 0928-4931
VL - 29
SP - 2085
EP - 2091
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
IS - 7
ER -