TY - JOUR
T1 - Pioneer glutamatergic cells develop into a morpho-functionally distinct population in the juvenile CA3 hippocampus
AU - Marissal, Thomas
AU - Bonifazi, Paolo
AU - Picardo, Michel Aimé
AU - Nardou, Romain
AU - Petit, Ludovic Franck
AU - Baude, Agnès
AU - Fishell, Gordon James
AU - Ben-Ari, Yehezkel
AU - Cossart, Rosa
N1 - Funding Information:
We thank Pr David Anderson for providing the Ngn2CreERTM mouse. We thank Corinne Brouchoud, Sherihane Bensemmane, Sarah Feldt Muldoon, Alexandre Jaouen, Philippe Guigue and Thomas Tressard for help with the experiments and analysis. Research in the Cossart group was supported by grants from the European Research Council (ERC FP7 Young Investigators #242852), the Fondation pour la Recherche Medicale (F.R.M.), the Neuron-ERANET project EPINet cofunded by the Agence Nationale pour la Recherche and the European Union Commission, the Fondation Bettencourt Schueller, I.N.S.E.R.M., the Ville de Marseille and Region P.A.C.A. and the F.R.C. Drs R.C. and A.B. are funded by the C.N.R.S. T.M. was funded by a fellowship from the Ministère de l’Education Nationale et de la Recherche and the F.R.M. Research in the Fishell laboratory is supported by the National Institutes of Health (NIH RO1 grants R01MH071679 and R01NS039007).
PY - 2012
Y1 - 2012
N2 - The developing CA3 hippocampus is comprised by highly connected hub neurons that are particularly effective in achieving network synchronization. Functional hub neurons were shown to be exclusively GABAergic, suggesting that the contribution of glutamatergic neurons to physiological synchronization processes at early postnatal stages is minimal. However, without fast GABAergic transmission, a different situation may prevail. In the adult CA3, blocking fast GABAergic transmission induces the generation of network bursts that can be triggered by the stimulation of single pyramidal neurons. Here we revisit the network function of CA3 glutamatergic neurons from a developmental viewpoint, without fast GABAergic transmission. We uncover a sub-population of early-generated glutamatergic neurons that impacts network dynamics when stimulated in the juvenile hippocampus. Additionally, this population displays characteristic morpho-physiological features in the juvenile and adult hippocampus. Therefore, the apparently homogeneous glutamatergic cell population likely displays a morpho-functional diversity rooted in temporal embryonic origins.
AB - The developing CA3 hippocampus is comprised by highly connected hub neurons that are particularly effective in achieving network synchronization. Functional hub neurons were shown to be exclusively GABAergic, suggesting that the contribution of glutamatergic neurons to physiological synchronization processes at early postnatal stages is minimal. However, without fast GABAergic transmission, a different situation may prevail. In the adult CA3, blocking fast GABAergic transmission induces the generation of network bursts that can be triggered by the stimulation of single pyramidal neurons. Here we revisit the network function of CA3 glutamatergic neurons from a developmental viewpoint, without fast GABAergic transmission. We uncover a sub-population of early-generated glutamatergic neurons that impacts network dynamics when stimulated in the juvenile hippocampus. Additionally, this population displays characteristic morpho-physiological features in the juvenile and adult hippocampus. Therefore, the apparently homogeneous glutamatergic cell population likely displays a morpho-functional diversity rooted in temporal embryonic origins.
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U2 - 10.1038/ncomms2318
DO - 10.1038/ncomms2318
M3 - Article
C2 - 23271650
AN - SCOPUS:84871738872
SN - 2041-1723
VL - 3
JO - Nature communications
JF - Nature communications
M1 - 1316
ER -