PKR is not required for interferon-γ inhibition of VSV replication in neurons

David A. Chesler; Jorge Luis Muñoz-Jordán; Nicola Donelan; Adolfo García-Sastre; Carol Shoshkes Reiss

doi:10.1089/088282403763635474

PKR is not required for interferon-γ inhibition of VSV replication in neurons

David A. Chesler, Jorge Luis Muñoz-Jordán, Nicola Donelan, Adolfo García-Sastre, Carol Shoshkes Reiss

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

In this report, the contribution of PKR to the IFN-γ mediated inhibition of VSV replication in neurons was examined. IFN-γ treatment of NB41A3 murine neuroblastoma cells resulted in the reduced expression of VSV protein during infection. PKR was found to be modestly upregulated in NB41A3 cells following IFN-γ treatment. The phosphorylation state of PKR and its downstream target, eIF2α, were unaffected by either IFN-γ or VSV infection. Inhibition of PKR through the use of 2-aminopurine or the expression of the Influenza A NS1 gene had no effect on the ability of IFN-γ to inhibit the replication of VSV in vitro. These data indicate that endogenously expressed PKR is not required for the IFN-γ mediated inhibition of VSV replication in NB41A3 neuroblastoma cells.

Original language	English (US)
Pages (from-to)	87-96
Number of pages	10
Journal	Viral Immunology
Volume	16
Issue number	1
DOIs	https://doi.org/10.1089/088282403763635474
State	Published - 2003

ASJC Scopus subject areas

Immunology
Molecular Medicine
Virology

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title = "PKR is not required for interferon-γ inhibition of VSV replication in neurons",

abstract = "In this report, the contribution of PKR to the IFN-γ mediated inhibition of VSV replication in neurons was examined. IFN-γ treatment of NB41A3 murine neuroblastoma cells resulted in the reduced expression of VSV protein during infection. PKR was found to be modestly upregulated in NB41A3 cells following IFN-γ treatment. The phosphorylation state of PKR and its downstream target, eIF2α, were unaffected by either IFN-γ or VSV infection. Inhibition of PKR through the use of 2-aminopurine or the expression of the Influenza A NS1 gene had no effect on the ability of IFN-γ to inhibit the replication of VSV in vitro. These data indicate that endogenously expressed PKR is not required for the IFN-γ mediated inhibition of VSV replication in NB41A3 neuroblastoma cells.",

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AU - Chesler, David A.

AU - Muñoz-Jordán, Jorge Luis

AU - Donelan, Nicola

AU - García-Sastre, Adolfo

AU - Reiss, Carol Shoshkes

PY - 2003

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N2 - In this report, the contribution of PKR to the IFN-γ mediated inhibition of VSV replication in neurons was examined. IFN-γ treatment of NB41A3 murine neuroblastoma cells resulted in the reduced expression of VSV protein during infection. PKR was found to be modestly upregulated in NB41A3 cells following IFN-γ treatment. The phosphorylation state of PKR and its downstream target, eIF2α, were unaffected by either IFN-γ or VSV infection. Inhibition of PKR through the use of 2-aminopurine or the expression of the Influenza A NS1 gene had no effect on the ability of IFN-γ to inhibit the replication of VSV in vitro. These data indicate that endogenously expressed PKR is not required for the IFN-γ mediated inhibition of VSV replication in NB41A3 neuroblastoma cells.

AB - In this report, the contribution of PKR to the IFN-γ mediated inhibition of VSV replication in neurons was examined. IFN-γ treatment of NB41A3 murine neuroblastoma cells resulted in the reduced expression of VSV protein during infection. PKR was found to be modestly upregulated in NB41A3 cells following IFN-γ treatment. The phosphorylation state of PKR and its downstream target, eIF2α, were unaffected by either IFN-γ or VSV infection. Inhibition of PKR through the use of 2-aminopurine or the expression of the Influenza A NS1 gene had no effect on the ability of IFN-γ to inhibit the replication of VSV in vitro. These data indicate that endogenously expressed PKR is not required for the IFN-γ mediated inhibition of VSV replication in NB41A3 neuroblastoma cells.

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PKR is not required for interferon-γ inhibition of VSV replication in neurons

Abstract

ASJC Scopus subject areas

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