PKR is not required for interferon-γ inhibition of VSV replication in neurons

David A. Chesler, Jorge Luis Muñoz-Jordán, Nicola Donelan, Adolfo García-Sastre, Carol Shoshkes Reiss

Research output: Contribution to journalArticlepeer-review

Abstract

In this report, the contribution of PKR to the IFN-γ mediated inhibition of VSV replication in neurons was examined. IFN-γ treatment of NB41A3 murine neuroblastoma cells resulted in the reduced expression of VSV protein during infection. PKR was found to be modestly upregulated in NB41A3 cells following IFN-γ treatment. The phosphorylation state of PKR and its downstream target, eIF2α, were unaffected by either IFN-γ or VSV infection. Inhibition of PKR through the use of 2-aminopurine or the expression of the Influenza A NS1 gene had no effect on the ability of IFN-γ to inhibit the replication of VSV in vitro. These data indicate that endogenously expressed PKR is not required for the IFN-γ mediated inhibition of VSV replication in NB41A3 neuroblastoma cells.

Original languageEnglish (US)
Pages (from-to)87-96
Number of pages10
JournalViral Immunology
Volume16
Issue number1
DOIs
StatePublished - 2003

ASJC Scopus subject areas

  • Immunology
  • Molecular Medicine
  • Virology

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