Plasmid-chromosome shuffling for non-deletion alleles in yeast

Zhiwei Huang, Richard S. Sucgang, Yu Yi Lin, Xiaomin Shi, Jef D. Boeke, Xuewen Pan

Research output: Contribution to journalArticlepeer-review


Here we describe a facile plasmid-chromosome shuffling technique for generating and analyzing non-deletion alleles in the yeast Saccharomyces cerevisiae. This technique takes advantage of an existing set of genome-wide haploid-convertible heterozygous diploid yeast knockout mutants. This simple method will facilitate characterization of essential gene functions and genome-wide investigation of protein structure-function relationships.

Original languageEnglish (US)
Pages (from-to)167-169
Number of pages3
JournalNature methods
Issue number2
StatePublished - Feb 2008

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Plasmid-chromosome shuffling for non-deletion alleles in yeast'. Together they form a unique fingerprint.

Cite this