Plasmid-chromosome shuffling for non-deletion alleles in yeast

Zhiwei Huang, Richard S. Sucgang, Yu Yi Lin, Xiaomin Shi, Jef D. Boeke, Xuewen Pan

    Research output: Contribution to journalArticlepeer-review


    Here we describe a facile plasmid-chromosome shuffling technique for generating and analyzing non-deletion alleles in the yeast Saccharomyces cerevisiae. This technique takes advantage of an existing set of genome-wide haploid-convertible heterozygous diploid yeast knockout mutants. This simple method will facilitate characterization of essential gene functions and genome-wide investigation of protein structure-function relationships.

    Original languageEnglish (US)
    Pages (from-to)167-169
    Number of pages3
    JournalNature methods
    Issue number2
    StatePublished - Feb 2008

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Molecular Biology
    • Cell Biology


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