Plasmid-chromosome shuffling for non-deletion alleles in yeast

Zhiwei Huang; Richard S. Sucgang; Yu Yi Lin; Xiaomin Shi; Jef D. Boeke; Xuewen Pan

doi:10.1038/nmeth.1173

Plasmid-chromosome shuffling for non-deletion alleles in yeast

Zhiwei Huang, Richard S. Sucgang, Yu Yi Lin, Xiaomin Shi, Jef D. Boeke, Xuewen Pan

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Here we describe a facile plasmid-chromosome shuffling technique for generating and analyzing non-deletion alleles in the yeast Saccharomyces cerevisiae. This technique takes advantage of an existing set of genome-wide haploid-convertible heterozygous diploid yeast knockout mutants. This simple method will facilitate characterization of essential gene functions and genome-wide investigation of protein structure-function relationships.

Original language	English (US)
Pages (from-to)	167-169
Number of pages	3
Journal	Nature methods
Volume	5
Issue number	2
DOIs	https://doi.org/10.1038/nmeth.1173
State	Published - Feb 2008

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1173

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title = "Plasmid-chromosome shuffling for non-deletion alleles in yeast",

abstract = "Here we describe a facile plasmid-chromosome shuffling technique for generating and analyzing non-deletion alleles in the yeast Saccharomyces cerevisiae. This technique takes advantage of an existing set of genome-wide haploid-convertible heterozygous diploid yeast knockout mutants. This simple method will facilitate characterization of essential gene functions and genome-wide investigation of protein structure-function relationships.",

author = "Zhiwei Huang and Sucgang, {Richard S.} and Lin, {Yu Yi} and Xiaomin Shi and Boeke, {Jef D.} and Xuewen Pan",

note = "Funding Information: We thank S. Kron (University of Chicago) for advice in implementing the 96-well yeast plasmid recovery protocol. This work was supported by a Baylor College of Medicine seed grant to X.P. and US National Institutes of Health (NIH) Roadmap grant {\textquoteleft}{\textquoteleft}Technology Center for Networks and Pathways{\textquoteright}{\textquoteright} (RR020839) to J.D.B. R.S.S. is supported by NIH P01 grant HD39691.",

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AU - Boeke, Jef D.

AU - Pan, Xuewen

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Plasmid-chromosome shuffling for non-deletion alleles in yeast

Abstract

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