Plasmid-chromosome shuffling for non-deletion alleles in yeast

Zhiwei Huang, Richard S. Sucgang, Yu Yi Lin, Xiaomin Shi, Jef D. Boeke, Xuewen Pan

    Research output: Contribution to journalArticle

    Abstract

    Here we describe a facile plasmid-chromosome shuffling technique for generating and analyzing non-deletion alleles in the yeast Saccharomyces cerevisiae. This technique takes advantage of an existing set of genome-wide haploid-convertible heterozygous diploid yeast knockout mutants. This simple method will facilitate characterization of essential gene functions and genome-wide investigation of protein structure-function relationships.

    Original languageEnglish (US)
    Pages (from-to)167-169
    Number of pages3
    JournalNature methods
    Volume5
    Issue number2
    DOIs
    StatePublished - Feb 2008

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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  • Cite this

    Huang, Z., Sucgang, R. S., Lin, Y. Y., Shi, X., Boeke, J. D., & Pan, X. (2008). Plasmid-chromosome shuffling for non-deletion alleles in yeast. Nature methods, 5(2), 167-169. https://doi.org/10.1038/nmeth.1173