Polyester modification of the mammalian trpm8 channel protein: Implications for structure and function

Chike Cao, Yevgen Yudin, Yann Bikard, Wei Chen, Tong Liu, Hong Li, Dieter Jendrossek, Alejandro Cohen, Evgeny Pavlov, Tibor Rohacs, Eleonora Zakharian

Research output: Contribution to journalArticle

Abstract

The TRPM8 ion channel is expressed in sensory neurons and is responsible for sensing environmental cues, such as cold temperatures and chemical compounds, including menthol and icilin. The channel functional activity is regulated by various physical and chemical factors and is likely to be preconditioned by its molecular composition. Our studies indicate that the TRPM8 channel forms a structural-functional complex with the polyester poly-(R)-3-hydroxybutyrate (PHB). We identified by mass spectrometry a number of PHB-modified peptides in the Nterminus of the TRPM8 protein and in its extracellular S3-S4 linker. Removal of PHB by enzymatic hydrolysis and site-directed mutagenesis of both the serine residues that serve as covalent anchors for PHB and adjacent hydrophobic residues that interact with the methyl groups of the polymer resulted in significant inhibition of TRPM8 channel activity. Weconclude that the TRPM8 channel undergoes posttranslational modification by PHB and that this modification is required for its normal function

Original languageEnglish (US)
Pages (from-to)302-315
Number of pages14
JournalCell Reports
Volume4
Issue number2
DOIs
StatePublished - Jul 25 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Cao, C., Yudin, Y., Bikard, Y., Chen, W., Liu, T., Li, H., Jendrossek, D., Cohen, A., Pavlov, E., Rohacs, T., & Zakharian, E. (2013). Polyester modification of the mammalian trpm8 channel protein: Implications for structure and function. Cell Reports, 4(2), 302-315. https://doi.org/10.1016/j.celrep.2013.06.022