TY - JOUR
T1 - Porphyrin-based photocatalytic nanolithography
T2 - A new fabrication tool for protein arrays
AU - Bearinger, Jane P.
AU - Stone, Gary
AU - Dugan, Lawrence C.
AU - El Dasher, Bassem
AU - Stockton, Cheryl
AU - Conway, James W.
AU - Kuenzler, Tobias
AU - Hubbell, Jeffrey A.
PY - 2009
Y1 - 2009
N2 - Nanoarray fabrication is a multidisciplinary endeavor encompassing materials science, chemical engineering, and biology. We formed nanoarrays via a new technique, porphyrin-based photocatalytic nanolithography. The nanoarrays, with controlled features as small as 200 nm, exhibited regularly ordered patterns and may be appropriate for (a) rapid and parallel proteomics screening of immobilized biomolecules, (b) protein-protein interactions, and/or (c) biophysical and molecular biology studies involving spatially dictated ligand placement. We demonstrated protein immobilization utilizing nanoarrays fabricated via photocatalytic nanolithography on silicon substrates where the immobilized proteins are surrounded by a non-fouling polymer background. Molecular & Cellular Proteomics 8:1823-1831, 2009.
AB - Nanoarray fabrication is a multidisciplinary endeavor encompassing materials science, chemical engineering, and biology. We formed nanoarrays via a new technique, porphyrin-based photocatalytic nanolithography. The nanoarrays, with controlled features as small as 200 nm, exhibited regularly ordered patterns and may be appropriate for (a) rapid and parallel proteomics screening of immobilized biomolecules, (b) protein-protein interactions, and/or (c) biophysical and molecular biology studies involving spatially dictated ligand placement. We demonstrated protein immobilization utilizing nanoarrays fabricated via photocatalytic nanolithography on silicon substrates where the immobilized proteins are surrounded by a non-fouling polymer background. Molecular & Cellular Proteomics 8:1823-1831, 2009.
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U2 - 10.1074/mcp.M800585-MCP200
DO - 10.1074/mcp.M800585-MCP200
M3 - Article
C2 - 19406753
AN - SCOPUS:71049165567
SN - 1535-9476
VL - 8
SP - 1823
EP - 1831
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
IS - 8
ER -