@article{3ade44c358034dc6a212af35000e96c0,
title = "Positioning a multifunctional basic helix-loop-helix transcription factor within the Ciona notochord gene regulatory network",
abstract = "In a multitude of organisms, transcription factors of the basic helix-loop-helix (bHLH) family control the expression of genes required for organ development and tissue differentiation. The functions of different bHLH transcription factors in the specification of nervous system and paraxial mesoderm have been widely investigated in various model systems. Conversely, the knowledge of the role of these regulators in the development of the axial mesoderm, the embryonic territory that gives rise to the notochord, and the identities of their target genes, remain still fragmentary. Here we investigated the transcriptional regulation and target genes of Bhlh-tun1, a bHLH transcription factor expressed in the developing Ciona notochord as well as in additional embryonic territories that contribute to the formation of both larval and adult structures. We describe its possible role in notochord formation, its relationship with the key notochord transcription factor Brachyury, and suggest molecular mechanisms through which Bhlh-tun1 controls the spatial and temporal expression of its effectors.",
keywords = "Brachyury, Ciona, Enhancer, Gene regulatory network, Notochord, bHLH",
author = "Kugler, {Jamie E.} and Yushi Wu and Lavanya Katikala and Passamaneck, {Yale J.} and Jermyn Addy and Natalia Caballero and Izumi Oda-Ishii and Maguire, {Julie E.} and Raymond Li and {Di Gregorio}, Anna",
note = "Funding Information: We acknowledge Dr. Nori Satoh and members of his lab (Okinawa Institute of Science and Technology) for the microarray screens. We thank Drs. William Smith and Shota Chiba (University of California, Santa Barbara) for the mutant Ci-Bra embryos. We are grateful to Mr. Joseph Afzali and Ms. Irina Pyatigorskaya for technical help, and to Drs. S{\'e}bastien Darras and Florian Razy-Krajka for insightful discussion. This work was supported by the National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS R01GM100466) and by start-up funds from NYU College of Dentistry to ADG. Funding Information: We acknowledge Dr. Nori Satoh and members of his lab (Okinawa Institute of Science and Technology) for the microarray screens. We thank Drs. William Smith and Shota Chiba (University of California, Santa Barbara) for the mutant Ci-Bra embryos. We are grateful to Mr. Joseph Afzali and Ms. Irina Pyatigorskaya for technical help, and to Drs. S{\'e}bastien Darras and Florian Razy-Krajka for insightful discussion. This work was supported by the National Institutes of Health/National Institute of General Medical Sciences ( NIH / NIGMS R01GM100466 ) and by start-up funds from NYU College of Dentistry to ADG. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = apr,
day = "15",
doi = "10.1016/j.ydbio.2019.01.002",
language = "English (US)",
volume = "448",
pages = "119--135",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",
}