Post-Assembly Stabilization of Rationally Designed DNA Crystals

Jiemin Zhao; Arun Richard Chandrasekaran; Qian Li; Xiang Li; Ruojie Sha; Nadrian C. Seeman; Chengde Mao

doi:10.1002/anie.201503610

Post-Assembly Stabilization of Rationally Designed DNA Crystals

Jiemin Zhao, Arun Richard Chandrasekaran, Qian Li, Xiang Li, Ruojie Sha, Nadrian C. Seeman, Chengde Mao

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

This manuscript reports an effort to stabilize self-assembled DNA crystals. Owing to their weak inter-unit cohesion, self-assembled DNA crystals are fragile, which limits the potential applications of such crystals. To overcome this problem, another molecule was introduced, which binds to the cohesive sites and stabilizes the inter-unit interactions. The extra interactions greatly improve the stability of the DNA crystals. The original DNA crystals are only stable in solutions of high ionic strength (e.g., ≥1.2 M (NH₄)₂SO₄); in contrast, the stabilized crystals can be stable at ionic strengths as low as that of a 0.02M solution of (NH₄)₂SO₄. The current strategy is expected to represent a general approach for increasing the stability of self-assembled DNA nanostructures for potential applications, for example, as structural scaffolds and molecular sieves.

Original language	English (US)
Pages (from-to)	9936-9939
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	54
Issue number	34
DOIs	https://doi.org/10.1002/anie.201503610
State	Published - Aug 1 2015

Keywords

DNA structures
crystal engineering
nanostructures
self-assembly

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201503610

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title = "Post-Assembly Stabilization of Rationally Designed DNA Crystals",

abstract = "This manuscript reports an effort to stabilize self-assembled DNA crystals. Owing to their weak inter-unit cohesion, self-assembled DNA crystals are fragile, which limits the potential applications of such crystals. To overcome this problem, another molecule was introduced, which binds to the cohesive sites and stabilizes the inter-unit interactions. The extra interactions greatly improve the stability of the DNA crystals. The original DNA crystals are only stable in solutions of high ionic strength (e.g., ≥1.2 M (NH4)2SO4); in contrast, the stabilized crystals can be stable at ionic strengths as low as that of a 0.02M solution of (NH4)2SO4. The current strategy is expected to represent a general approach for increasing the stability of self-assembled DNA nanostructures for potential applications, for example, as structural scaffolds and molecular sieves.",

keywords = "DNA structures, crystal engineering, nanostructures, self-assembly",

author = "Jiemin Zhao and Chandrasekaran, {Arun Richard} and Qian Li and Xiang Li and Ruojie Sha and Seeman, {Nadrian C.} and Chengde Mao",

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AU - Zhao, Jiemin

AU - Chandrasekaran, Arun Richard

AU - Li, Qian

AU - Li, Xiang

AU - Sha, Ruojie

AU - Seeman, Nadrian C.

AU - Mao, Chengde

PY - 2015/8/1

Y1 - 2015/8/1

N2 - This manuscript reports an effort to stabilize self-assembled DNA crystals. Owing to their weak inter-unit cohesion, self-assembled DNA crystals are fragile, which limits the potential applications of such crystals. To overcome this problem, another molecule was introduced, which binds to the cohesive sites and stabilizes the inter-unit interactions. The extra interactions greatly improve the stability of the DNA crystals. The original DNA crystals are only stable in solutions of high ionic strength (e.g., ≥1.2 M (NH4)2SO4); in contrast, the stabilized crystals can be stable at ionic strengths as low as that of a 0.02M solution of (NH4)2SO4. The current strategy is expected to represent a general approach for increasing the stability of self-assembled DNA nanostructures for potential applications, for example, as structural scaffolds and molecular sieves.

AB - This manuscript reports an effort to stabilize self-assembled DNA crystals. Owing to their weak inter-unit cohesion, self-assembled DNA crystals are fragile, which limits the potential applications of such crystals. To overcome this problem, another molecule was introduced, which binds to the cohesive sites and stabilizes the inter-unit interactions. The extra interactions greatly improve the stability of the DNA crystals. The original DNA crystals are only stable in solutions of high ionic strength (e.g., ≥1.2 M (NH4)2SO4); in contrast, the stabilized crystals can be stable at ionic strengths as low as that of a 0.02M solution of (NH4)2SO4. The current strategy is expected to represent a general approach for increasing the stability of self-assembled DNA nanostructures for potential applications, for example, as structural scaffolds and molecular sieves.

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KW - self-assembly

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Post-Assembly Stabilization of Rationally Designed DNA Crystals

Abstract

Keywords

ASJC Scopus subject areas

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