TY - JOUR
T1 - Posttranscriptional Regulation of Neuronal Nitric Oxide Synthase Expression by IFN-γ
AU - Chesler, David A.
AU - McCutcheon, Jane A.
AU - Reiss, Carol Shoshkes
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/2
Y1 - 2004/2
N2 - In this report, the mechanism through which interferon-γ (IFN-γ) regulates the expression of nitric oxide synthase (NOS-1) in neurons was examined. We have shown previously that IFN-γ treatment of cells results in a two log inhibition of vesicular stomatitis virus (VSV) production. This inhibition of VSV replication is dependent both in vitro and in vivo on nitric oxide (NO) production by NOS-1. Furthermore, this effect is associated with the increased expression and activity of NOS-1 following IFN-γ treatment. In vitro, exposure to IFN-γ prior to infection with VSV is a prerequisite to establish an effective antiviral state, indicating the necessity for a priming event. Neuroblastoma cells (NB41A3) were treated with IFN-γ or medium and examined for changes in NOS-1 protein and mRNA expression. NOS-1 protein expression was found to be increased after IFN-γ treatment, and this was associated with increases in both neosynthesis and NOS-1 protein stability. NOS-1 transcription and mRNA levels were unaffected by IFN-γ treatment. These data demonstrate that IFN-γ regulates NOS-1 expression through posttranscriptional and posttranslational mechanisms.
AB - In this report, the mechanism through which interferon-γ (IFN-γ) regulates the expression of nitric oxide synthase (NOS-1) in neurons was examined. We have shown previously that IFN-γ treatment of cells results in a two log inhibition of vesicular stomatitis virus (VSV) production. This inhibition of VSV replication is dependent both in vitro and in vivo on nitric oxide (NO) production by NOS-1. Furthermore, this effect is associated with the increased expression and activity of NOS-1 following IFN-γ treatment. In vitro, exposure to IFN-γ prior to infection with VSV is a prerequisite to establish an effective antiviral state, indicating the necessity for a priming event. Neuroblastoma cells (NB41A3) were treated with IFN-γ or medium and examined for changes in NOS-1 protein and mRNA expression. NOS-1 protein expression was found to be increased after IFN-γ treatment, and this was associated with increases in both neosynthesis and NOS-1 protein stability. NOS-1 transcription and mRNA levels were unaffected by IFN-γ treatment. These data demonstrate that IFN-γ regulates NOS-1 expression through posttranscriptional and posttranslational mechanisms.
UR - http://www.scopus.com/inward/record.url?scp=1342330621&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1342330621&partnerID=8YFLogxK
U2 - 10.1089/107999004322813381
DO - 10.1089/107999004322813381
M3 - Article
C2 - 14980078
AN - SCOPUS:1342330621
SN - 1079-9907
VL - 24
SP - 141
EP - 149
JO - Journal of Interferon and Cytokine Research
JF - Journal of Interferon and Cytokine Research
IS - 2
ER -