POU6f1 mediates neuropeptide-dependent plasticity in the adult brain

Cynthia K. McClard, Mikhail Y. Kochukov, Isabella Herman, Zhandong Liu, Aiden Eblimit, Yalda Moayedi, Joshua Ortiz-Guzman, Daniel Colchado, Brandon Pekarek, Sugi Panneerselvam, Graeme Mardon, Benjamin R. Arenkiel

Research output: Contribution to journalArticlepeer-review


The mouse olfactory bulb (OB) features continued, activity-dependent integration of adult-born neurons, providing a robust model with which to examine mechanisms of plasticity in the adult brain.Wepreviously reported that localOBinterneurons secrete the neuropeptide corticotropin-releasing hormone (CRH) in an activity-dependent manner onto adult-born granule neurons and that local CRH signaling promotes expression of synaptic machinery in the bulb. This effect is mediated via activation of the CRH receptor 1 (CRHR1), which is developmentally regulated during adult-born neuron maturation. CRHR1 is a G S -protein-coupled receptor that activates CREBdependent transcription in the presence of CRH. Therefore, we hypothesized that locally secreted CRH activates CRHR1 to initiate circuit plasticity programs. To identify such programs, we profiled gene expression changes associated with CRHR1 activity in adult-born neurons of the OB. Here, we show that CRHR1 activity influences expression of the brain-specific Homeobox-containing transcription factor POU Class 6 Homeobox 1 (POU6f1). To elucidate the contributions of POU6f1 toward activity-dependent circuit remodeling, we targeted CRHR1 + neurons in male and female mice for cell-type-specific manipulation of POU6f1 expression. Whereas loss of POU6f1 in CRHR1 + neurons resulted in reduced dendritic complexity and decreased synaptic connectivity, overexpression of POU6f1 in CRHR1 + neurons promoted dendritic outgrowth and branching and influenced synaptic function. Together, these findings suggest that the transcriptional program directed by POU6f1 downstream of local CRH signaling in adult-born neurons influences circuit dynamics in response to activity-dependent peptide signaling in the adult brain.

Original languageEnglish (US)
Pages (from-to)1443-1461
Number of pages19
JournalJournal of Neuroscience
Issue number6
StatePublished - Feb 7 2018


  • Adult-born neurons
  • Crh
  • Development
  • Neuropeptides
  • Olfactory
  • Synapse

ASJC Scopus subject areas

  • General Neuroscience


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