Power in numbers: Single-cell RNA-seq strategies to dissect complex tissues

Research output: Contribution to journalReview articlepeer-review


The growing scale and declining cost of single-cell RNA-sequencing (RNA-seq) now permit a repetition of cell sampling that increases the power to detect rare cell states, reconstruct developmental trajectories, and measure phenotype in new terms such as cellular variance. The characterization of anatomy and developmental dynamics has not had an equivalent breakthrough since groundbreaking advances in live fluorescent microscopy. The new resolution obtained by single-cell RNA-seq is a boon to genetics because the novel description of phenotype offers the opportunity to refine gene function and dissect pleiotropy. In addition, the recent pairing of high-throughput genetic perturbation with single-cell RNA-seq has made practical a scale of genetic screening not previously possible.

Original languageEnglish (US)
Pages (from-to)203-221
Number of pages19
JournalAnnual Review of Genetics
StatePublished - Nov 23 2018


  • pooled CRISPR
  • rare cell types
  • single-cell RNA-seq
  • single-cell power analysis
  • trajectory analysis

ASJC Scopus subject areas

  • Genetics


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