Power tools for gene expression and clonal analysis in Drosophila

Alberto Del Valle Rodríguez, Dominic Didiano, Claude Desplan

Research output: Contribution to journalReview article

Abstract

The development of two-component expression systems in Drosophila melanogaster, one of the most powerful genetic models, has allowed the precise manipulation of gene function in specific cell populations. These expression systems, in combination with site-specific recombination approaches, have also led to the development of new methods for clonal lineage analysis. We present a hands-on user guide to the techniques and approaches that have greatly increased resolution of genetic analysis in the fly, with a special focus on their application for lineage analysis. Our intention is to provide guidance and suggestions regarding which genetic tools are most suitable for addressing different developmental questions.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalNature methods
Volume9
Issue number1
DOIs
StatePublished - Jan 2012

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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