Power tools for gene expression and clonal analysis in Drosophila

Alberto Del Valle Rodríguez; Dominic Didiano; Claude Desplan

doi:10.1038/nmeth.1800

Power tools for gene expression and clonal analysis in Drosophila

Alberto Del Valle Rodríguez, Dominic Didiano, Claude Desplan

Research output: Contribution to journal › Review article › peer-review

Abstract

The development of two-component expression systems in Drosophila melanogaster, one of the most powerful genetic models, has allowed the precise manipulation of gene function in specific cell populations. These expression systems, in combination with site-specific recombination approaches, have also led to the development of new methods for clonal lineage analysis. We present a hands-on user guide to the techniques and approaches that have greatly increased resolution of genetic analysis in the fly, with a special focus on their application for lineage analysis. Our intention is to provide guidance and suggestions regarding which genetic tools are most suitable for addressing different developmental questions.

Original language	English (US)
Pages (from-to)	47-55
Number of pages	9
Journal	Nature methods
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/nmeth.1800
State	Published - Jan 2012

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1800

Cite this

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title = "Power tools for gene expression and clonal analysis in Drosophila",

abstract = "The development of two-component expression systems in Drosophila melanogaster, one of the most powerful genetic models, has allowed the precise manipulation of gene function in specific cell populations. These expression systems, in combination with site-specific recombination approaches, have also led to the development of new methods for clonal lineage analysis. We present a hands-on user guide to the techniques and approaches that have greatly increased resolution of genetic analysis in the fly, with a special focus on their application for lineage analysis. Our intention is to provide guidance and suggestions regarding which genetic tools are most suitable for addressing different developmental questions.",

author = "{Del Valle Rodr{\'i}guez}, Alberto and Dominic Didiano and Claude Desplan",

note = "Funding Information: We thank X. Li, C. Bertet, N. Vogt, D. Vasiliauskas, J. Rister and R.J. Johnston for helpful comments on the manuscript. A.d.V.R. is funded by a fellowship from Fundaci{\'o}n Caja Madrid. D.D. is funded by a US National Institutes of Health postdoctoral fellowship 1F32EY021065. This work was supported by a grant from US National Institutes of Health EY13012 to C.D.",

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Power tools for gene expression and clonal analysis in Drosophila

Abstract

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