@article{e7f51f9e6fd1416eaac2c41cfbbbb023,
title = "Precise control of SCRaMbLE in synthetic haploid and diploid yeast",
abstract = "Compatibility between host cells and heterologous pathways is a challenge for constructing organisms with high productivity or gain of function. Designer yeast cells incorporating the Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) system provide a platform for generating genotype diversity. Here we construct a genetic AND gate to enable precise control of the SCRaMbLE method to generate synthetic haploid and diploid yeast with desired phenotypes. The yield of carotenoids is increased to 1.5-fold by SCRaMbLEing haploid strains and we determine that the deletion of YEL013W is responsible for the increase. Based on the SCRaMbLEing in diploid strains, we develop a strategy called Multiplex SCRaMbLE Iterative Cycling (MuSIC) to increase the production of carotenoids up to 38.8-fold through 5 iterative cycles of SCRaMbLE. This strategy is potentially a powerful tool for increasing the production of bio-based chemicals and for mining deep knowledge.",
author = "Bin Jia and Yi Wu and Li, {Bing Zhi} and Mitchell, {Leslie A.} and Hong Liu and Shuo Pan and Juan Wang and Zhang, {Hao Ran} and Nan Jia and Bo Li and Michael Shen and Xie, {Ze Xiong} and Duo Liu and Cao, {Ying Xiu} and Xia Li and Xiao Zhou and Hao Qi and Boeke, {Jef D.} and Yuan, {Ying Jin}",
note = "Funding Information: This work was funded by the National Natural Science Foundation of China (21750001, 21621004, and 21676192), the Ministry of Science and Technology of China ({"}973{"} Program, 2014CB745100), and the International S&T Cooperation Program of China (2015DFA00960). We thank Hui-Min Liu and Zi-Xi Chen for help on mating type switching of synX yeast and flow cytometer experiments. We thank Wen-Hai Xiao and Ming-Zhu Ding for help on transcriptome analysis and helpful discussion. Funding Information: This work was funded by the National Natural Science Foundation of China (21750001, 21621004, and 21676192), the Ministry of Science and Technology of China (“973” Program, 2014CB745100), and the International S&T Cooperation Program of China (2015DFA00960). We thank Hui-Min Liu and Zi-Xi Chen for help on mating type switching of synX yeast and flow cytometer experiments. We thank Wen-Hai Xiao and Ming-Zhu Ding for help on transcriptome analysis and helpful discussion. Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = dec,
day = "1",
doi = "10.1038/s41467-018-03084-4",
language = "English (US)",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}