PRINCIPLES AND APPLICATIONS OF FLUORESCENCE TECHNIQUES IN BIOPHYSICAL CHEMISTRY

Research output: Contribution to journalArticle

Abstract

Abstract— Fluorescence is widely used as a probe of the properties of ligand‐biopolymer complexes or adducts, proteins, nucleic acids and membranes. The principles and techniques utilized in characterizing the basic fluorescence properties of a system of fluorophore‐biopolymer complexes are outlined. The different approaches utilized include a correlation of fluorescence decay profiles obtained by time‐correlated single photon counting techniques with (1)steady‐state fluorescence excitation and emission spectra, (2)relative fluorescence quantum yields, (3)fluorophore‐accessibilities investigated by quenching techniques, and (4)ligand‐macromolecule binding equilibria.

Original languageEnglish (US)
Pages (from-to)547-553
Number of pages7
JournalPhotochemistry and photobiology
Volume45
Issue number4
DOIs
StatePublished - Apr 1987

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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