@article{d4f960e2da08425ea78be13023db4b92,
title = "Programmed Supramolecular Assemblies Using Orthogonal Pairs of Heterodimeric Coiled Coil Peptides",
abstract = "Despite recent progress, it remains challenging to program biomacromolecules to assemble into discrete nanostructures with pre-determined sizes and topologies. We report here a novel strategy to address this challenge. By using two orthogonal pairs of heterodimeric coiled coils as the building blocks, we constructed six discrete supramolecular assemblies, each composed of a prescribed number of coiled coil components. Within these assemblies, different coiled coils were connected via end-to-side covalent linkages strategically pre-installed between the non-complementary pairs. The overall topological features of two highly complex assemblies, a “barbell” and a “quadrilateral” form, were characterized experimentally and were in good agreement to the designs. This work expands the design paradigms for peptide-based discrete supramolecular assemblies and will provide a route for de novo fabrication of functional protein materials.",
keywords = "Coiled Coils, Heterodimers, Nanostructures, Peptides, Supramolecular Assemblies, α-Helices",
author = "Linhai Jiang and Xiaobing Zuo and Jianping Li and Traaseth, {Nathaniel J.} and Kent Kirshenbaum",
note = "Funding Information: This study was supported by award CHE‐2002890 from the National Science Foundation (NSF). L.J. would like to gratefully acknowledge the New York University Chemistry Department for the Margaret and Herman Sokol Fellowship. N.J.T. acknowledges support from NIH R01AI108889. The authors would like to thank Dr. Shahid Khan for the assistance on SEC purifications. Dr. Jia Ma from the Chemistry Department of Columbia University is greatly appreciated for the assistance on performing the SEC‐MALS analysis. This work was supported in part through the NYU IT High Performance Computing resources, services, and staff expertise. Joseph Sall, Dr. Feng‐Xia (Alice) Liang and Dr. William J. Rice from NYU School of Medicine are greatly acknowledged for assistance with TEM imaging. The authors acknowledge the contribution of James Eastwood for assistance with this manuscript and Prof. Vikas Nanda (Rutgers University) for helpful discussions. The Circular Dichroism Spectropolarimeter used in this work was acquired through the support of New York University. SAXS measurements were performed at Beamline 12‐ID‐B of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE‐AC02‐06CH11357. Publisher Copyright: {\textcopyright} 2022 Wiley-VCH GmbH.",
year = "2022",
doi = "10.1002/anie.202201895",
language = "English (US)",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
}