Progress on developing wavefront sensorless adaptive optics optical coherence tomography for in vivo retinal imaging in mice

Azhar Zam, Pengfei Zhang, Yifan Jian, Marinko V. Sarunic, Stefano Bonora, Edward N. Pugh, Robert J. Zawadzki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a new design for a wavefront sensorless adaptive optics (WS-AO) Fourier domain optical coherence tomography (FD-OCT) system for small animal retinal imaging in vivo. Without the optical complications necessary for inclusion of a wavefront sensor in the optical system, this version of WS-AO FD-OCT system has a simplified optical design, including elimination of long focal length scanning optics and optical conjugation of vertical and horizontal scanners. This modification provides a modular large Field of View for retinal screening (25 degree visual angle), while also allowing a "zoom" capability for allocating all the scanning resources to a smaller region of interest, allowing high resolution aberration-corrected imaging. In the present system we used a 0 Dpt contact lens to stabilize the mouse eye position and to allow long duration imaging. Defocus (axial focus position) in our system is controlled by the collimation of the OCT sample arm entrance beam.

Original languageEnglish (US)
Title of host publicationOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX
EditorsValery V. Tuchin, James G. Fujimoto, Joseph A. Izatt
PublisherSPIE
ISBN (Electronic)9781628414028
DOIs
StatePublished - 2015
EventOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX - San Francisco, United States
Duration: Feb 8 2015Feb 11 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9312
ISSN (Print)1605-7422

Conference

ConferenceOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX
Country/TerritoryUnited States
CitySan Francisco
Period2/8/152/11/15

Keywords

  • Adaptive optics
  • Animal models
  • Imaging system
  • Medical optics instrumentation
  • Ophthalmology
  • Optical coherence tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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