@article{395262bd7cd843078262f827fde27533,
title = "Prospective study of oral microbiome and gastric cancer risk among Asian, African American and European American populations",
abstract = "Colonization of specific bacteria in the human mouth was reported to be associated with gastric cancer risk. However, previous studies were limited by retrospective study designs and low taxonomic resolutions. We performed a prospective case-control study nested within three cohorts to investigate the relationship between oral microbiome and gastric cancer risk. Shotgun metagenomic sequencing was employed to characterize the microbiome in prediagnostic buccal samples from 165 cases and 323 matched controls. Associations of overall microbial richness and abundance of microbial taxa, gene families and metabolic pathways with gastric cancer risk were evaluated via conditional logistic regression. Analyses were performed within each cohort, and results were combined by meta-analyses. We found that overall microbial richness was associated with decreased gastric cancer risk, with an odds ratio (OR) per standard deviation (SD) increase in Simpson's reciprocal index of 0.77 (95% confidence interval [CI] = 0.61-0.99). Nine taxa, 38 gene families and six pathways also showed associations with gastric cancer risk at P <.05. Neisseria mucosa and Prevotella pleuritidis were enriched, while Mycoplasma orale and Eubacterium yurii were depleted among cases with ORs and 95% CIs per SD increase in centered log-ratio transformed taxa abundance of 1.31 (1.03-1.67), 1.26 (1.00-1.57), 0.74 (0.59-0.94) and 0.80 (0.65-0.98), respectively. The top two gene families (P = 3.75 × 10−4 and 3.91 × 10−4) and pathways (P = 1.75 × 10−3 and 1.53 × 10−3) associated with gastric cancer were related to the decreased risk and are involved in hexitol metabolism. Our study supports the hypothesis that oral microbiota may play a role in gastric cancer etiology.",
author = "Yaohua Yang and Jirong Long and Cong Wang and Blot, {William J.} and Zhiheng Pei and Xiang Shu and Fen Wu and Nathaniel Rothman and Jie Wu and Qing Lan and Qiuyin Cai and Wei Zheng and Yu Chen and Shu, {Xiao Ou}",
note = "Funding Information: The authors wish to thank study participants and research team members who have enabled this work to be carried out. We thank Regina Courtney, Dr Hui Cai and Dr Mary Shannon Byers for their help with sample preparation, data management, technical support and editorial assistance for this project. This project was supported by the National Cancer Institute (NCI) grant R01CA204113. Parent studies, the SMHS, the SWHS and the SCCS, were supported by National Institutes of Health (NIH) grants UM1CA173640, UM1CA182910 and U01CA202979, respectively. The data analyses were conducted using the Advanced Computing Center for Research and Education (ACCRE) at Vanderbilt University. Sample preparation was conducted at the Survey and Biospecimen Shared Resources, which is supported in part by the Vanderbilt-Ingram Cancer Center (P30 CA68485). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the study. Funding Information: The authors wish to thank study participants and research team members who have enabled this work to be carried out. We thank Regina Courtney, Dr Hui Cai and Dr Mary Shannon Byers for their help with sample preparation, data management, technical support and editorial assistance for this project. This project was supported by the National Cancer Institute (NCI) grant R01CA204113. Parent studies, the SMHS, the SWHS and the SCCS, were supported by National Institutes of Health (NIH) grants UM1CA173640, UM1CA182910 and U01CA202979, respectively. The data analyses were conducted using the Advanced Computing Center for Research and Education (ACCRE) at Vanderbilt University. Sample preparation was conducted at the Survey and Biospecimen Shared Resources, which is supported in part by the Vanderbilt‐Ingram Cancer Center (P30 CA68485). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the study. Publisher Copyright: {\textcopyright} 2021 UICC.",
year = "2022",
month = mar,
day = "15",
doi = "10.1002/ijc.33847",
language = "English (US)",
volume = "150",
pages = "916--927",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "6",
}