Prostaglandins E2 and F enhance differentiation of cementoblastic cells

P. M. Camargo, R. Lagos, F. Q.M. Pirih, A. Benitez, J. M. Nervina, Sotirios Tetradis

Research output: Contribution to journalArticle

Abstract

Background: The prostaglandins (PG) E2 and PGF are important cytokines in periodontal physiology and pathology. PGE2 and PGF alter cell function by binding and activating the plasma-membrane G-protein-coupled PG receptors. In this study, we examined the PGE2 and PGF effects on the immortalized cementoblastic OCCM cells. Methods: Confluent OCCM cells were treated with PGE2, PGF, specific activators/inhibitors of the EP prostanoid receptors, a specific activator of the FP prostanoid receptor, and direct activators/inhibitors of the protein kinase C (PKC) signaling pathway. Mineral nodule formation was assessed by the von Kossa stain. Results: PGE2 and PGF significantly increased mineralization of OCCM cells. The EP1 and EP3 PG receptor activators 16,16-dimethyl-prostagiandin E2 and sulprostone, also increased mineralization. In contrast, specific activators of the EP2 or the EP2/EP3/EP4 receptors did not have any effect. Fluprostenol, a specific activator of the FP receptor, significantly increased mineralization of OCCM cells. FP and EP (1 or 3) receptors signal through activation of the protein kinase C (PKC) pathway. Indeed, phorbol 12-myristate 13-acetate (PMA), a direct activator of the PKC pathway, significantly increase OCCM mineralization, while pre-treatment of OCCM cells with the PKC inhibitor GF109203x bisindolylmaleimide) significantly decreased mineralization. Conclusion: We conclude that PGE2 and PGF exert an anabolic effect on OCCM mineralization through activation of PKC signaling.

Original languageEnglish (US)
Pages (from-to)303-309
Number of pages7
JournalJournal of periodontology
Volume76
Issue number2
DOIs
StatePublished - Feb 2005

Keywords

  • Cells, OCCM
  • Cementogenesis
  • Dental cementum
  • Prostaglandin E
  • Prostaglandin F
  • Protein kinase C
  • Receptors, EP
  • Receptors, FP
  • Receptors, prostaglandin

ASJC Scopus subject areas

  • Periodontics

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    Camargo, P. M., Lagos, R., Pirih, F. Q. M., Benitez, A., Nervina, J. M., & Tetradis, S. (2005). Prostaglandins E2 and F enhance differentiation of cementoblastic cells. Journal of periodontology, 76(2), 303-309. https://doi.org/10.1902/jop.2005.76.2.303