Protein abundances are more conserved than mRNA abundances across diverse taxa

Jon M. Laurent, Christine Vogel, Taejoon Kwon, Stephanie A. Craig, Daniel R. Boutz, Holly K. Huse, Kazunari Nozue, Harkamal Walia, Marvin Whiteley, Pamela C. Ronald, Edward M. Marcotte

Research output: Contribution to journalArticle

Abstract

Proteins play major roles in most biological processes; as a consequence, protein expression levels are highly regulated. While extensive post-transcriptional, translational and protein degradation control clearly influence protein concentration and functionality, it is often thought that protein abundances are primarily determined by the abundances of the corresponding mRNAs. Hence surprisingly, a recent study showed that abundances of orthologous nematode and fly proteins correlate better than their corresponding mRNA abundances. We tested if this phenomenon is general by collecting and testing matching large-scale protein and mRNA expression data sets from seven different species: two bacteria, yeast, nematode, fly, human, and rice. We find that steady-state abundances of proteins show significantly higher correlation across these diverse phylogenetic taxa than the abundances of their corresponding mRNAs (p=0.0008, paired Wilcoxon). These data support the presence of strong selective pressure to maintain protein abundances during evolution, even when mRNA abundances diverge.

Original languageEnglish (US)
Pages (from-to)4209-4212
Number of pages4
JournalProteomics
Volume10
Issue number23
DOIs
StatePublished - Dec 2010

Keywords

  • Global protein analysis
  • Protein expression
  • Quantitative analysis
  • Shotgun proteomics
  • Systems biology

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Laurent, J. M., Vogel, C., Kwon, T., Craig, S. A., Boutz, D. R., Huse, H. K., Nozue, K., Walia, H., Whiteley, M., Ronald, P. C., & Marcotte, E. M. (2010). Protein abundances are more conserved than mRNA abundances across diverse taxa. Proteomics, 10(23), 4209-4212. https://doi.org/10.1002/pmic.201000327