Protein Acetylation Microarray Reveals that NuA4 Controls Key Metabolic Target Regulating Gluconeogenesis

Yu yi Lin, Jin ying Lu, Junmei Zhang, Wendy Walter, Weiwei Dang, Jun Wan, Sheng Ce Tao, Jiang Qian, Yingming Zhao, Jef D. Boeke, Shelley L. Berger, Heng Zhu

Research output: Contribution to journalArticlepeer-review


Histone acetyltransferases (HATs) and histone deacetylases (HDACs) conduct many critical functions through nonhistone substrates in metazoans, but only chromatin-associated nonhistone substrates are known in Saccharomyces cerevisiae. Using yeast proteome microarrays, we identified and validated many nonchromatin substrates of the essential nucleosome acetyltransferase of H4 (NuA4) complex. Among these, acetylation sites (Lys19 and 514) of phosphoenolpyruvate carboxykinase (Pck1p) were determined by tandem mass spectrometry. Acetylation at Lys514 was crucial for enzymatic activity and the ability of yeast cells to grow on nonfermentable carbon sources. Furthermore, Sir2p deacetylated Pck1p both in vitro and in vivo. Loss of Pck1p activity blocked the extension of yeast chronological life span caused by water starvation. In human hepatocellular carcinoma (HepG2) cells, human Pck1 acetylation and glucose production were dependent on TIP60, the human homolog of ESA1. Our findings demonstrate a regulatory function for the NuA4 complex in glucose metabolism and life span by acetylating a critical metabolic enzyme.

Original languageEnglish (US)
Pages (from-to)1073-1084
Number of pages12
Issue number6
StatePublished - Mar 20 2009



ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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