@article{20d77769fe944bbd85eae87ec2d046d4,
title = "Protein Engineered Triblock Polymers Composed of Two SADs: Enhanced Mechanical Properties and Binding Abilities",
abstract = " Recombinant methods have been used to engineer artificial protein triblock polymers composed of two different self-assembling domains (SADs) bearing one elastin (E) flanked by two cartilage oligomeric matrix protein coiled-coil (C) domains to generate CEC. To understand how the two C domains improve small molecule recognition and the mechanical integrity of CEC, we have constructed C L44A EC L44A , which bears an impaired C L44A domain that is unstructured as a negative control. The CEC triblock polymer demonstrates increased small molecule binding and ideal elastic behavior for hydrogel formation. The negative control C L44A EC L44A does not exhibit binding to small molecule and is inelastic at lower temperatures, affirming the favorable role of C domain and its helical conformation. While both CEC and C L44A EC L44A assemble into micelles, CEC is more densely packed with C domains on the surface enabling the development of networks leading to hydrogel formation. Such protein engineered triblock copolymers capable of forming robust hydrogels hold tremendous promise for biomedical applications in drug delivery and tissue engineering.",
author = "Olsen, {Andrew J.} and Priya Katyal and Haghpanah, {Jennifer S.} and Kubilius, {Matthew B.} and Ruipeng Li and Schnabel, {Nicole L.} and O'Neill, {Sean C.} and Yao Wang and Min Dai and Navjot Singh and Tu, {Raymond S.} and Montclare, {Jin Kim}",
note = "Funding Information: This work was supported by the ARO W911NF-15-1-0304 (J.K.M.) and in part by the NSF MRSEC Program under Award DMR-1420073, the Society of Plastic Engineers (J.S.H.), NIH-RISE R25GM056833-16 (S.O.) NSF-DMR 1006407 (M.K.), and GK-12 Fellows Grant DGE-0741714 (J.S.H.). This research used CMS beamline of the National Synchrotron Light Source II, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Brookhaven National Laboratory under Contract DE-SC0012704. We thank Dr. Kevin Yager and Dr. Dmytro Nykypanchuk for assistance with SAXS experiments. Funding Information: This work was supported by the ARO W911NF-15-1-0304 (J.K.M.) and in part by the NSF MRSEC Program under Award DMR-1420073, the Society of Plastic Engineers (J.S.H.), NIH-RISE R25GM056833-16 (S.O.), NSF-DMR 1006407 (M.K.), and GK-12 Fellows Grant DGE-0741714 (J.S.H.). This research used CMS beamline of the National Synchrotron Light Source II, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Brookhaven National Laboratory under Contract DESC0012704. We thank Dr. Kevin Yager and Dr. Dmytro Nykypanchuk for assistance with SAXS experiments. We are grateful to NYULMC DART Microscopy Lab for TEM. We also thank Dr. Jeffrey F. Morris and Mr. Omer Sedes of the Chemical Engineering Department, City College of New York, for assistance with the rheology experiments. Publisher Copyright: Copyright {\textcopyright} 2018 American Chemical Society.",
year = "2018",
month = may,
day = "14",
doi = "10.1021/acs.biomac.7b01259",
language = "English (US)",
volume = "19",
pages = "1552--1561",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "5",
}