Protein surface recognition by synthetic agents: Design and structural requirements of a family of artificial receptors that bind to cytochrome c

Qing Lin, Hyung Soon Park, Yoshitomo Hamuro, Chang Sun Lee, Andrew D. Hamilton

Research output: Contribution to journalArticle

Abstract

The design, synthesis, and evaluation of a novel series of receptors for protein surface recognition are described. The design of these agents is based around the attachment of four constrained peptide loops onto a central calix[4]arene scaffold. This arrangement mimics the role of the hypervariable loops in antibody combining regions and defines a large surface area for binding to a complementary region of the exterior of a target protein. Using affinity and gel filtration chromatographies we show that one particular receptor binds strongly to the surface of cytochrome c. The site of binding is presumably close to the heme edge region, which contains several charged lysine residues. This is supported by the observation that the receptor inhibits the reduction of Fe(III) cytochrome c to its Fe(II) form. We also show that binding is strongly dependent on the nature of the substitutents on the lower rim of the calixarene. The nmr and computational studies suggest that this effect may be due to conformational differences among the differently substituted receptors.

Original languageEnglish (US)
Pages (from-to)285-297
Number of pages13
JournalBiopolymers - Peptide Science Section
Volume47
Issue number4
DOIs
StatePublished - 1998

Keywords

  • Artificial receptors
  • Cytochrome c
  • Protein surface recognition

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

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